Electrochemical Sensor Base On Molecularly Imprinted To Detect Cancerogen In Food

As a worldwide challenge for human health,food safety has attracted wide attention.In order to solve food safety problems,many methods have been developed to detect carcinogenic contaminants in food.Among these approaches,the molecularly imprinted electrochemical sensor shows the advantages of high selectivity,stability,rapid,sensitive,low cost and simple operation.In this paper,two molecularly imprinted electrochemical sensors were prepared to detect two typical carcinogens that may exist in food,and satisfactory results were obtained.The research of this paper includes:1.Using bisphenol A glycidyl ether?BADGE?as template and pyrrole as functional monomer,the molecularly imprinted electrochemical sensor MIP-PDE that sensitive to BADGE was prepared on platinum-plate electrode?PDE?with electropolymerization and elution processes.K₃Fe?CN?₆/K₄Fe?CN?₆solution was used as electroactive probe to indirectly detect BADGE.The modification of MIP was verified by scanning electron microscopy?SEM?,Micro-FTIR,cyclic voltammetry?CV?and Electrochemical Impedance Spectroscopy?EIS?.The optimal conditions for the preparation of MIP-PDE were investigated.Under the optimal conditions,the prepared MIP-PDE sensor showed good sensitivity within the concentration range of 0.05-10.00?mol dm^-3,and the detection limit was 0.04?mol dm^-3.The sensor had excellent selectivity,stability and reproducibility and had been successfully used for the determination of BADGE in edible oil samples.2.Zeolite imidazolate framework-8?ZIF-8?and ZIF-8 derived nanoporous carbon?ZC?were prepared.CV,EIS,SEM and XRD was used to characterize ZIF-8 and ZC.The results showed that the prepared ZC had high electrical conductivity and stable porous structure,high specific surface area,and excellent electrochemical activity.Using Tetrabutyl hydroquinone?TBHQ?as template molecule and o-phenylenediamine?o-PD?as functional monomer,the molecularly imprinted electrochemical sensor MIP/ZC/GCE was constructed based on ZC modified glass-carbon electrode?ZC/GCE?.And SEM was used to characterize the morphology of MIP/ZC/GCE,which proved that MIP polymerized on the surface of ZC particles uniformly.The preparation conditions of MIP/ZC/GCE were optimized.Under the best conditions,the prepared MIP/ZC/GCE was used to detect TBHQ,showing a good linear relationship in the range of 1.0-75?mol dm^-3,and the detection limit was 0.42?mol dm^-3.MIP/ZC/GCE show not only excellent reproducibility and stability,but also good selectivity.Detection results of TBHQ in real oil samples were satisfactory.