Preparation And Application Of Glucose Sensor Based On Non-precious Metal Materials

Accurate detection of glucose is necessary and significant in the current era that diabetes is outbreaking.Among the existing detection methods,the electrochemical method has been proved to be fast,easy to operate and highly sensitive.However,the catalytic performance of the materials,the stability of the sensors and the application of the whole systems still need to be further improved and developed.In this thesis,we developed four types of electrochemical glucose sensors with excellent performancethrough the synthesis of new materials with highly catalytic capablility according to the rapid hydrothermal reaction and the electrochemical self-assembly modification.The main contents are summarized as follows:?1?We synthesized three-dimensional nitrogen-doped porous graphene hydrogel nanocomposites doped by Ni Co₂O₄?3D NHGH/Ni Co₂O₄?using a simple one-pot hydrothermal method.The characterizations such as specific surface area measurement,scanning electron microscopy,and X-ray diffraction revealed the as-synthesized materials with excellent conductivity,large specific surface area and rich active sites,enabling its good electrocatalytic detection.The nanocomposites-modified electrode exihibited good performance for the determination of glucose and hydrogen peroxide?H₂O₂?.?2?Based on the hydrothermal method,the pure octahedral Cu-metal organic skeleton?HUSK-1,Cu-MOF?crystals were prepared.The carbon paste electrode containing Cu-MOF was applied for the efficient determination of glucose after one step full mixing.The resulting glucose sensor has good sensitivity,stability with a limit of detection?LOD?as low as 0.11?M and a linear range as broad as 5?M-10.95 m M.In addition,the measurement of blood samples were also conducted.?3?A novel non-enzymatic glucose sensor was developed based on the electrodes modified with the carbon nanotubes and Cu-MOF multilayer films,which was self-assembled via electrodeposition method.The sensor preparation is easy to operate and effective by simply using a constant potential method,which can overcome the disadvantage of the traditional electrode modification method.After optimizing the parameters,a wide detection range of 0.5?M-11.84 m M was obtained,and the selectivity,reproducibility,and determination of actual blood samples were also studied.?4?We developed small flexible electrochemical sensors using laser-engraved graphene?LSG?electrodes,which were prepared via a direct laser engraving process on the polyimide?PI?films.In comparison to the traditional solid electrode,the structure of LSG electrodes can be easily tuned,and the flexible electrochemical glucose sensors were constructed.The prepared LSG eletrode play a good role in enzyme fixation,and under the action of Pt nanoparticles,the glucose biosensor with good comprehensive performance was constructed and successfully applied to the detection of blood samples with satisfactory results.