Correlation Analysis Of Transcription Metabolism And Changes Of Micronutrients Controlled By Light Signal Of Mung Bean Sprouts

Mung bean is a traditional economic crop in China.This thesis investigated the metabolic regulation of vitamin C and polyphenols during the legume development of mung bean,and the metabolic regulation network of mung bean sprout was analyzed by multiple omics.Moreover,the metabolism of light regulation to strengthen the nutritional quality of mung bean sprouts was explored.The main results are as follows:(1)The content of ASA,gallic acid,daidzein and total phenol was highest in 8-DAF during mung bean legume development,and the synergistic effect of VTC2 and GME was essential for the increase of ASA content at 8-DAF and 17-DAF.Importantly,the genes expression of PAL,CHS,and IFS were positively correlated with daidzein,daidzein,and total phenol content.(2)The correlation analysis of transcriptome and metabolomics in the morphogenesis of mung bean showed that 124 pathways,including cell division,environmental stress,genetic information process and metabolic regulation,were significantly regulated.Pathways were involving plant hormone signal regulation,one carbon metabolism,TCA cycle,starch sucrose metabolism and amino acid biosynthesis.Furtherly,we found that GA3 and IAA played an important role in early seed germination of mung bean.(3)Germination significantly increased the content of vitamin C,folate,carotenoids and vitamin E in mung beans.The content of these vitamins in mung bean sprouts was several times higher than mung bean seeds.Light can promote the accumulation of vitamins to a certain extent,especially the photoperiod condition.ASA,folate,carotenoids,and vitamin E(?-tocopherol)had the highest contents in 6-DAST,which were 9.5,6.3,15.4,and 2.6(25.9)times of the initial content of mung bean,respectively.(4)The key enzyme genes of vitamins metabolism were very important;they controlled the vitamin levels and improved the nutritional quality of plants.The light signal had an obvious regulatory effect on metabolism of vitamin C,folate,ASA,vitamin E and carotenoid.The expression of key genes of vitamin C metabolism,including PMI,GME,GLDH,Gal UR,DHAR and AO,were closely related to changes of vitamin C content during light-responsive mung bean germination.The key genes of the folate metabolism,including GTPCH,ADCS,HPPK,and DHFR,were sensitive to light response and regulated folate.PDS and CHYB had effects on light response and zeaxanthin synthesis,LCYB is the key gene for ?-carotene synthesis and the expressions of LUT5,LUT1 and ZE were related to light regulated lutein synthesis.The key genes of vitamin E metabolism,including HPT,TC and TMT,were related to the significant increase of ?-tocopherol under photoperiod conditions.In addition,there were interactions between vitamins,under semi-light and constant dark conditions.ASA,folate,vitamin E(?-tocopherol)and carotenoids had synergistic effect,while vitamin C inhibited the accumulation of vitamin E and carotenoids under constant light condition.