Construction And Application Of Novel Fluorecent Systems Based On Aggregation-induced Emission Materials

With the rapid development of the tumor marker analysis and detection industry in recent years,many detection methods for different types of markers have been born.It is especially important to find a method with low background signal and high sensitivity.The concept of aggregation-induced emission materials was first proposed in 2001,and its unique fluorescent properties provided a new way of thinking for the construction of novel fluorescent systems.At this stage,aggregation-induced emission materials have shown great application value in areas such as fluorescence analysis,photoelectric sensing,and medical clinical diagnosis.A variety of aggregation-induced emission materials have been designed and synthesized in this thesis.Based on this,new fluorescence systems are established for the research of tumor marker detection,which provides new ideas and platforms for clinical auxiliary diagnosis.The main research contents are summarized as follows:A zinc ion(Zn²⁺)-triggered aggregation induced emission enhancement?AIEE? fluorescence“on-off-on”nanoswitch was fabricated for inorganic pyrophosphate?PPi? and inorganic pyrophosphatase?PPase?activity detection.Dual ligand functionalized Au NCs were utilized as the substrate of the AIEE nanoswitch.The introduction of Zn²⁺can cause Au NCs aggregated along with the enhanced fluorescence.After the addition of PPi,aggregated Au NCs disaggregated along with decreased fluorescence due to the competitive combination between PPi and Zn²⁺?on-off?.When PPase was introduced,PPi was hydrolyzed and release Zn²⁺,resulting in aggregated Au NCs along with enhanced fluorescence again?off-on?.On the basis of this,highly selective and sensitive detection PPi?liner range from 0.1??M to 300??M?and PPase activity?liner range from 0.1 mU/mL to 10 mU/mL?can be achieved.The detection limits are0.04??M for PPi and 0.03 mU/mL for PPase,respectively.Furthermore,the as-prepared Zn²⁺-triggered AIEE nanoswitch was successfully used for quantitative analysis of PPase activity in human serum,and applied for the inhibitors screening.Afterwards,with tetraphenylethylene?TPE?as the core,three water-soluble aggregation-induced emission?AIE?organic molecular probes?TPE-NRs?were designed and synthesized in this thesis.We found that these three different probes produced different fluorescence responses to ten different proteins.Based on this phenomenon,we used these three AIE probes as sensing units to build a new type of fluorescent sensing array.The principal component analysis was used to analyze and process the cross-response signal generated by the array and the protein to achieve the purpose of distinguishing ten proteins..We also used Autodock software to simulate the binding process of proteins and probe molecules,and thoroughly discussed and verified the mechanism of generating different fluorescent signals.Because the aggregation-induced luminescent material has the advantage of low background signal,the array sensor has a high sensitivity,and can distinguish 10?g/mL proteins in 10 minutes.In addition,because the content of various proteins in the serum of different cancer patients is different,the array sensor can be used for the initial diagnosis and identification of cancer,and the receiver operating characteristic curve?ROC curve?is used to evaluate the diagnostic effect of the sensor,showing its potential applications in rapid clinical diagnosis.