Visible/Near Infrared Absorbing Organic Dyes For Up-Conversion Light-Induced Releasing And Bioorthogonal Labeling

A better treatment of cancer has become the concer of researchers,with the continuous development of science and technology in the field of biochemistry.Light can induce drug release and also be used as a treatment.Since the photodynamic therapy was proposed,this method has been proveed to possess many advantages over traditional cancer treatment.For example,photodynamic therapy can achieve targeted therapy and at the same time can better control the treatment process.The hypoxic environment inside the tumor will affect the efficacy of photodynamic therapy.Most tumors grow inside the organism,and the depth of treatment is also an urgent problem to be solved.The target of this paper is to design a strategy to improve the efficacy of photodynamic therapy in hypoxic environment.What can not be ignored is that increasing the depth of treatment is also very important.The main research contents for the above issues are as follows:(1)Many improvement programs improve the treatment effect by changing the hypoxic environment inside the tumor.However,traditional schemes cannot solve the problem of treatment limitation.In this paper,Ru(bpy)3 and DNCP derivatives(DNCP-F,DNCP-Me,DNCP-Me O)are combined into three molecular groups.Photodynamic therapy and small molecule therapy are combined by triplet-triplet annihilation(TTA)up-conversion release process.The main goal is to achieve photodynamic therapy under aerobic conditions,and carbon monoxide molecular therapy dominates after oxygen is depleted.The design process above was realized in vitro,through UV-Vis absorption spectroscopy and nuclear magnetic resonance spectroscopy test analysis.Further,reaction kinetics calculations are also very important characterization data.(2)Most traditional photosensitizers have insufficient treatment depth,which results in the reduce of treatment effect.In this paper,the tetrazine unit is introduced into the cyanine structure(Tz-Cy7).Cyanine is a near infrared dye,and some selected cyanines have phototherapy properties.The near infrared excitation light source will greatly increase the depth of tissue penetration.In addition,bioorthogonal reaction between tetrazine and norbornene in living body.Bioorthogonal reaction has the advantages of high selectivity and reactivity.It is important that the reaction does not affect the organism.More accurate phototherapy and biological imaging will be realized in the future.