Study On The Detection Of Three Opioids Based On SERS Technology

With the rapid development of medical treatment,opioids are often chosen to help patients effectively relieve pain.However,due to the transmission of these drugs through the nervous system and their binding with opioid receptors in the body,people will feel happy,so improper use will have a high risk of addiction.The abuse of opioids has become a serious problem in today's society.The criminal violence caused by it threatens people's life and property.The rapid detection and identification of these drugs can provide an effective means to prevent the illicit circulation and abuse of opioids.In this paper,SERS technology with high sensitivity,portability and simplicity,combined with two kinds of high-performance active substrates,was utilized to identify and semiquantitatively analyze the standards and biological samples of three opioids,i.e.dezocine,bucinnazine hydrochloride and fentanyl citrate,which is helpful to improve the supervision system and test data of analgesic drugs.This paper firstly introducesd the development and enhancement mechanism of SERS effect,and analyzed the research progress and application of SERS technology and substrate in opioid detection at home and abroad.Then,the density functional theory was expanded around the basic framework of density functional theory,and the main idea of multi-body problem in quantum mechanics by density functional theory was introduced.The molecular structures of the three opioids were optimized by using the B3 LYP mixed functional included in the Gaussin software,and the theoretical spectra of the three opioids were obtained by calculation.At the same time,the peak positions were assigned according to the vibration correspondence.Then the silver nano sol was synthetized by reduction reaction.The morphology,particle size distribution and absorption spectrum of the sol were characterized and sensitivity and uniformity of the substrate were explored by probe molecule 4-MBA.Based on the prepared silver sol and SERS,the standard aqueous solution of dezocine and bucinnazine hydrochloride,artificial urine and rat serum were determined qualitatively and semiquantitatively.According to the relationship between the fitted sample concentration and the peak value,the recovery efficiency of biological samples was measured to verify the accuracy of semiquantitative analysis.Finally,three kinds of gold nanospheres with different particle sizes were prepared by seed mediated growth combined with etching,and the gold nanospheres were assembled on the filter paper to synthesize the filter paper substrate by the way of interface self-assembly.And the assembly state,sensitivity,uniformity before and after etching and time stability were characterized.The paper-based substrate with the best particle size was selected,where the chloride ion was introduced to improve the enhance performance of the substrate.The semiquantitative analysis and recovery calculation of fentanyl citrate standard and biological samples were carried out by the optimized substrate.It can be proved that this method is fast and accurate by experimental results,and it is hopeful to be applied to the field real-time detection of opioids.