Preparation Of Blue-light Absorbing Inorganic Nanofilms And The Protective Effects For Human Retinal Pigment Epithelium Cells

With the popularization of various electronic products,electronic devices with screens as well as artificial illumination have become an important part of public life and work.As one of the three primary colors of light,blue light is indispensable in applications such as full-color display and in white lighting.Humans are exposed to increasing amounts of artificial blue-rich white light.However,it is becoming increasingly evident that exposure to this artificial light is hazardous to human eyes,especially for retinal pigment epithelium?RPE?cells.There are reports that the 415–455 nm short-wavelength blue-light range may be the most damaging for RPE cells.When the intensity and time of blue light break down the defense capability of the RPE cells,cell dysfunction and death will happen,and the cell damage is irreversible.Further,blue light also can induce the retinal disease development or progression such as age-related macular degeneration?AMD?and retinal pigment degeneration,and even lead to loss of vision.Hence,it is of great significance to develop materials and methods to reduce blue-light hazards efficiently while providing a comfortable visual experience,and further prove the protective effects of the blue-light filter from a biological point of view.Therefore,in this paper,based on the principle of semiconductor absorption,two blue-light shielding material system were developed,and achieved the purpose that filter the short-wavelength blue-light effectively,while maintaining high transmittance in the region with wavelengths greater than 500 nm.In addition,the protective effects of blue-light shielding filter on blue light induced damage of human retinal pigment epithelial cells?ARPE-19?were verified via cell experiments.The work in this thesis can be mainly listed as follows:?1?Preparation and characterization of Zn O/Cd O?ZC?composite films.Zn O/Cd O composite films was prepared by doping the Cd O into Zn O using sol-gel spin coating method.Furthermore,volume ratios of Zn/Cd were adjusted to change the absorption edge.In addition,UV treatment further improved the blue-light shielding performance for ZC films,and the reasons were discussed.The results indicate that when volume ratio of Zn/Cd is 3:5,the average cut-off rate in the region of 415-455 nm is 40.20%,meanwhile the average transmittance in the region greater than 500 nm reached 91.2%,and this filter was used for the following cell experiments.Finally,a blue light with a peak of 425 nm was used for blue light irradiation of ARPE-19 cells,and the changes of cell morphology results showed that the ZC thin films decreased the blue light induced ARPE-19 cells damage.?2?Preparation and optimization of Bi VO₄?BVO?thin films.Firstly,the effects of annealing temperature?300-600??were investigated on the blue light-shielding properties of BVO films.The UV-vis spectra results confirmed the 500?is the optimal annealing temperature,and the optimized BVO film could shield 49.86%short wavelength blue light?415-455 nm?,while maintaining greater than 87%average transmittance in the wavelength range of 500-800 nm.Secondly,the size of BVO nanoparticles was changed by adjusting the acetic acid/N,N-dimethylformamide?AA/DMF?ratio in the precursor solution,thus achieving different absorption of blue light for BVO films.Further,a double-film structure was designed with the thickness of 36.85 nm and 37.70 nm respectively and its blue light blocking rate increased to65.25%.Moreover,the damp heat test?85?,85%relative humidity?showed the excellent stability of the BVO films as their transmittances remained unchanged for 15d.In addition,the blue-light weighted radiance,L_B,decreased by 34.32%,along with slight changes in the average color rendering index?Ra?as well as the chromaticity coordinates of the International Commission on Illumination?CIE?;using this up-scaled large-area filter on a phone screen not only reduced blue-light hazards but also ensured satisfactory visual experience.?3?Protective effect of the BVO double-film on blue-light induced ARPE-19 cell damage.Cell experiments were performed to investigate the biological protective effects of the BVO filter on ARPE-19 cells.The results showed that the BVO double-film could significantly alleviate blue-light-induced cell impairments including morphological abnormality,reduction in cell viability,and migration ability.In addition,the application of the filter restored the decreased expression level of RPE functional indicators;at the same time,it downregulated the increased expression levels of inflammatory factors after light exposure.