Study On Key Mechanism Of Protein Transport Based On Nano-electrospraying

With the development of molecular science,people's understanding of diseases has reached the level of single molecules.To cure diseases from a single molecule level,the corresponding single-molecule drug processing technology is needed.As the bearer of life activities,protein plays an increasingly important role in the field of disease treatment.Protein nano-drug has also become an important part of nanomedicine,so the preparation of protein nanoparticles has also received extensive attention.In this paper,the molecular dynamics simulation and experimental research on the transport mechanism of nano-electrospraying technology in the preparation of protein nanoparticles are provided,which provides a basis for the application of electrostatic nano-spray in the field of protein.The main research results and contents are as follows:1)Study the characteristics of nano-electrospraying,which mainly includes startup voltage analysis and nanojet mode transition.It was found that the nano-electrospraying initiation field strength of KCl solution is about 0.8 V/nm.It is also found that the concentration of KCl solution does not affect the starting field strength of nano-electrospraying,but it has an effect on the spray mode of electrostatic nanojet.The higher the concentration of KCl solution,the easier it is to form a trickle.2)The morphological changes of proteins under the action of electric fields were analyzed.Mainly for the early exploration of protein electrostatic nano-spray,observe that the protein will be deformed and inactivated under the action of electric field.The simulation results show that the protein does not necessarily deform and deactivate under the action of the electric field,which is mainly related to the electric field strength and the time of the electric field.There is no significant change in protein morphology when the electric field strength is relatively small.When the electric field strength is large enough,the protein will unfold,and the degree of protein unfolding and the speed at which unfolding occurs are proportional to the field strength.3)Four different structures of proteins were selected from the RCSB PDB protein database to analyze their nano-electrospraying characteristics.It was found that the presence of protein caused a slight increase in the starting voltage of the electrostatic nanospray,mainly because the presence of protein reduced the field strength at the nano-nozzle resulting in insufficient electric field force to overcome the surface tension of the conductive liquid at the nozzle.It was found that the structure of two of the four proteins(6bx9,4uby)did not change significantly,and the spatial structure of the other two proteins(2jof,1zni)changed.This finding indicates that nano-electrospraying technology is suitable for the preparation of partial protein nanoparticles.4)The research on the transport mechanism in the preparation of protein particles based on electrostatic nanojet is not limited to the method of molecular dynamics simulation analysis,and the simulation results are also verified experimentally.In the experiment,the concentration of KCl solution affect the mode of nanospray and the starting voltage of the protein electrostatic nanojet is slightly higher than the starting voltage of the electrostatic nanojet was found.Moreover,the starting voltage of the electrostatic ejection is reduced by the nano-stage nozzle,so that the starting voltage of the protein electrostatic nano-jet is maintained at about 700 V.