Controllable Synthesis And Bioanalytical Applications Of Near-infrared Persistent Luminescence Nanoparticles

Persistent luminescence materials?PLM?can exhibit long-lived luminescence after the stoppage of excitation.In recent years,new persistent luminescence materials systems have developed rapidly.Various bottom-up and top-down methods of material synthesis have been developed and applied to the synthesis of persistent luminescence materials.Persistent luminescence materials have long afterglow duration and stable optical properties,which can effectively eliminate the autofluorescence interference,thus guiding high signal-to-noise ratio and high sensitivity of biological sensing and imaging.In addition,near-infrared persistent luminescence materials can significantly improve the imaging sensitivity,which is conducive to expanding its application in biomedicine.Biomedical applications require adjustable properties of nanomaterials,such as size and luminescent properties.However,controllable synthesis of persistent luminescence nanoparticles?PLNP?has rarely been reported,which greatly limits the development prospects of persistent luminescence materials.It is urgent to achieve the regulation of persistent luminescence nanomaterials.In this thesis,the persistent luminescence nanoparticles Zn₃Ga₂GeO₈:Cr?ZGGO:Cr?were synthesized by doping Ge in a typical near infrared emitting persistent luminescence materials ZnGa₂O₄:Cr.Then,the controlled synthesis of the persistent luminescence nanoparticles was realized by hydrothermal synthesis method.The nanoprobe based on persistent luminescence materials was further designed for the detection of biological small molecules.The main contents of this thesis are as follows:?1?Controllable synthesis of Zn₃Ga₂GeO₈:Cr?ZGGO:Cr?persistent luminescence nanoparticles.First,the size and luminescent properties of ZGGO:Cr PLNPs were adjusted by changing the pH value of the hydrothermal reaction system.Morever,the afterglow intensity of ZGGO:Cr PLNPs was effectively controlled by surface modification of electron-pushing and electron-absorbing groups.The ZGGO:Cr PLNPs have high luminescent intensity and long afterglow duration,which have good application prospects in background-free biosensor and bioimaging.?2?The ZGGO:Cr@MnO₂ nanoprobes were constructed for the detection of glutathione.The detection of glutathione was based on the principle of F?rster resonance energy transfer.The luminescence of ZGGO:Cr nanoparticles was quenched when the ZGGO:Cr nanoparticles were coated with manganese dioxide.After the reaction between manganese dioxide and glutathione,manganese dioxide was reduced and dissolved.The quantitative detection of glutathione can be achieved by detecting the luminescence intensity of the nanoprobe.Morever,the ZGGO:Cr@MnO₂ nanoprobes have high selectivity and sensitivity to glutathione.