Analytical Technologies For Hazardous-Chemical Detection In Honey And E'jiao

Honey and E'jiao are two important medicine and food dual purposes products.The safety of these two products have been widely concerned by consumers.This paper established a high-resolution mass spectrometers library for multiple potentially harmful compounds by ultraperformance liquid chromatography quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS).Then,the ultrahigh performance liquid chromatography-triple quadrod mass spectrometry(UPLC-MS/MS)methods were develoed for analysis of phytotoxins in honey and pesticides in Ejiao.Details are as follows:A comprehensive mass spectra based library of 590 chemical hazardous,including natural toxins,illegal adulterants,pesticides,veterinary drugs and contaminants,was constructed using UPLC-QTOF-MS,and then proper pretreatment procedures was considered for the preparation of honey and E'jiao.A preliminary QuEChERS pretreatment procedures was developed for rapid highthrough-put screening of chemicals.An UPLC-MS/MS mehod was estabolised for the simultaneous determination of 18 phytotoxins,including tripterygium alkaloids,pyrrolizidine alkaloids,isoquinolines,corianin and tutin,in honey.Acetonitrile extraction,QuEChERS and other pretreatment process were compared.Matrix-matched external standard calibration curve was utilized for quantification.Method validation included limit of detection(LOD),lower limit of quantitation(LLOQ),linearity,precision and recovery was evaluated.The method resulted in good linearity(R~2?0.99)for all of the phytotoxins and LOQ ranging from 0.002?g/L to 0.7?g/L except nuciferine and tutin is 2 and 4?g/L respectively.The mean recoveries of all detected compounds were 70~112%at three levels of spiked concentration,only the recoveries of lycopsamine was 48~60%.The method provides an efficient strategy for simultaneously qualitative analyzing multiple types phytotoxin in honey.An UPLC-MS/MS mehod was estabolised for the simultaneous determination of 281 pesticides in E'jiao.By comparing the sample pretreatment method of QuEChERS and comparing the purification effects of C18,PSA,Z-sep+and other adsorbents,Z-sep+was proved the optimal.Recoveries for 70%of the compounds were above 60%and the detection limit of 80%compounds was below 1?g/L.This method can satisfy the needs of high-throughput detection of pesticides in E'jiao.