Catalysis And Analysis Of Nuclease Mineralization Based On Metal-organic Frameworks In Non-aqueous Environments

DNAzymes have received great recent attention as a new class of artificial metalloenzymes based on DNA.Despite their promising catalytic performance,structural stability has limited the potential of DNAzymes.In this paper,we encapsulated G-Quadruplex-based DNAzymes into zeolite imidazolate backbone 90(ZIF-90)to form a DNAzyme-ZIF-90 composite through a biomimetic mineralization process.The structural stability of encapsulated DNAzymes is increased greatly,allowing DNAzymes retained high activity in the presence of Exonuclease I,organic solvents,or high temperature,which denatures free DNAzymes.Due to the increased stability and heterogeneous phase of this composite catalyst,we designed a continuous flow system to mimic its long-term stability in an industrial continuous flow system.Moreover,the catalytic activity of this composite catalyst can be reactivated by introducing K~+into the continuous-flow system.In addition,we use an example of asymmetric sulfonation of methylphenyl sulfide to demonstrate that DNAzymes can still maintain its high enantioselectivity after being encapsulated in metal-organic frameworks.This work provides a new strategy for stabilizing DNAzymes and expanding its application in the chemical industry.