Synthesis,Characterization And Properties Of Biopeptide Supramolecular Imprinted Membranes

Recent years,a new effective separation technique,molecular imprinting technique has been developed rapidly,also,It has a good application prospect in biomedicine,separation and purification,resource recycling and environmental detection.In this paper,amino acid molecularly imprinted polymers with chitosan as functional monomers and tuftsin molecularly imprinted polymers with acrylic acid,methacrylic acid and acrylamide as functional monomers were designed and synthesized by molecularly imprinting technology.The product structure was characterized,the synthesis technology was optimized,and various properties of molecularly imprinted polymers were investigated.The optimum preparation conditions of L-amino acid-chitosan molecularly imprinted composite membrane were studied by orthogonal experimental design.It was determined that the swelling property of molecularly imprinted composite membrane was better when the dosage of chitosan 2 g,PEG 2000 0.6 g and glutaraldehyde 0.2 mL were used.The properties of L-amino acid molecularly imprinted chitosan membrane were investigated by pure water flux,tensile strength and selectivity experiments.When the casting solution of the molecularly imprinted chitosan membrane was 5 g and the addition amount of L-amino acid was 0.1 g,the pure water flux and mechanical properties of the molecularly imprinted chitosan membrane were the best.The experiments showed that the separation factor of D,L-methionine selective permeability could reach 1.76.In the experiment,tuftsin was used as template molecule,and MAA,AA and am were used as functional monomers,By calculating the binding energy of tuftsin and functional monomers,the strength of H-bond formation between tuftsin and monomers was determined,and the best functional monomers of tuftsin were determined to be acrylic acid;In this paper,the best preparation conditions of molecularly imprinted membrane were determined by studying the morphology,pure water flux and mechanical properties of molecularly imprinted membrane.When the amount of methacrylic acid and glycol dimethacrylate was 1 mmol and 4 mmol respectively,and the polymerization temperature was 60 oC,the pure water flux and mechanical properties of molecularly imprinted membrane were the best.In this paper,tuftsin molecularly imprinted membrane was prepared by UVirradiation with tuftsin as template,Through pure water flux,mechanical properties,permeation capacity of molecularly imprinted membrane,equilibrium adsorption experiment and selectivity of molecularly imprinted membrane,the best ratio of functional monomers to prepare molecularly imprinted membrane was determined,When the functional monomer of molecularly imprinted membrane is AA and the ratio of imprinted molecule to functional monomer is 1:8,the mechanical properties and pure water flux of molecularly imprinted membrane are better;When the concentration of the adsorbent was 3 mg / ml and the temperature of the adsorbent was 298 K,the adsorption capacity of the molecularly imprinted membrane was the highest;The data analysis of the kinetic adsorption of molecularly imprinted membrane shows that the linear correlation coefficient of the quasi second-order kinetic equation is better,which confirms that the kinetic behavior of MIP conforms to the quasi second-order equation;the thermodynamic analysis of MIP on tuftsin shows that the thermodynamic behavior of tuftsin molecularly imprinted membrane conforms to Freundlich isotherm adsorption equation,which shows that the adsorption process is a process of chemical and physical adsorption.The selectivity of tuftsin AA molecularly imprinted membrane to tuftsin,cyclosporin A(CSA)and bovine serum albumin(BSA)was 23.08 %.This paper studies and optimizes the analysis and test conditions of tuftsin,establishes the standard curve of tuftsin,and obtains the best analysis and test method through the verification of methodology.In this paper,molecular imprinting technology is applied to the field of peptide supramolecular structure,and molecular simulation calculation is used to determine the best functional monomers.Tuftsin molecularly imprinted polymers with high adsorption capacity and good selection effect are prepared to realize the selective separation of tuftsin molecules.The study of this paper has certain significance for the separation and acquisition of tuftsin.