Preparation And Properties Of Chitosan Derivatives/Oxidized Sodium Alginate Hydrogels

In the process of wound treatment,reasonable protection of the wound surface can effectively promote wound healing.Therefore,the development of new wound dressing is essential for promoting wound healing.Chitosan derivatives,sodium alginate and curcumin have significant effects in the treatment of skin wounds.In this article,a new type of medical hydrogel material with good biocompatibility,nontoxicity and harmlessness was prepared by the crosslinking of chitosan derivatives and oxidized sodium alginate(OSA)and loading curcumin.The prepared hydrogel materials had good properties included moisture absorption,biocompatibility and significantly promoted wound healing.The aim is to provide a theory for developing novel medical wound dressings.The main research contents and results of this article are as follows:(1)Firstly,a water-soluble chitosan derivative carboxymethyl chitosan(CMC)was prepared,and oxidized sodium alginate was used as a macromolecular crosslinking agent.The different hydrogels(CMSA)were prepared by changing the amount of oxidized sodium alginate added.This hydrogel loaded curcumin complex to form carboxymethyl chitosan/oxidized sodium alginate-curcumin hydrogel(CMSA/CUR).The results of SEM showed that all hydrogel samples had a stable three-dimensional network structure.The shortest gel time was 102 s and the hydrogel had good swelling properties,and the maximum swelling ratio reached 235%.The hydrogel had proper water retention capacity.Cytotoxicity experiments showed that carboxymethyl chitosan/oxidized sodium alginate hydrogel had good biocompatibility and no toxic side effects on human skin fibroblasts.The addition of curcumin increased the biocompatibility of the hydrogel material and had a certain inhibitory effect on scar fibroblasts.(2)Firstly,a water-soluble hydroxypropyl chitosan(HPCS)was prepared,and oxidized sodium alginate was used as a macromolecular crosslinking agent.The different hydrogels(HPSA)were prepared by changing the amount of oxidized sodium alginate added.This hydrogel loaded curcumin complex to form hydroxypropyl chitosan/oxidized sodium alginate-curcumin hydrogel(HPSA/CUR).The results of SEM showed that all hydrogel samples had a stable three-dimensional network structure.The gel time was the shortest when the amount of oxidized sodium alginate added was 8 mL.The hydrogel showed good swelling properties and an appropriate water retention capacity.Cytotoxicity experiments showed that hydroxypropyl chitosan/oxidized sodium alginate hydrogel had good biocompatibility and no toxic side effects on human skin fibroblasts.The addition of curcumin increased the biocompatibility of the hydrogel material and had a certain inhibitory effect on scar fibroblasts.(3)The deep second degree burn wound model of SD rats was established.The animal experiment of hygrogels was to investigate the effect of the products on promoting the burn wound healing by wound general observation,wound healing efficacy,wound healing time and optical microscope observation.The results suggested that the effect of wound healing of CMSA/CUR and HPSA/CUR was better than CMSA and HPSA,and the both were better than control groups with regard to the wound general observation on the same time.Among them,the CMSA/CUR and HPSA/CUR had the best effect,and the wound healing time was(15±1)days and(16±0.5)days;the CMSA and HPSA were the second,the wound healing time was(18±1)days and(19±0.5)days;the lowest in the saline group,the wound healing time was(21±0.5)days.