Study On Anti-inflammatory And Antioxidant Effects Of Momordicin IIE In RAW 264.7 Cells

Momordica grosvenori is a valuable dual-use plant resource with edible and medicinal purposes,by exerting effects on treating sore throat,removing sputum,quenching thirst and relieving constipation.Mogroside V,the main bioactive component of Momordica grosvenori,has strong anti-inflammatory and antioxidant abilities both in vitro and vivo.Whether Mogroside IIE,as the main metabolite of Mogroside V in vivo,has the same biological activity remains to be explored.The main purpose of this subject is through advancedWestern blotting,enzyme-linked immunosorbent assay and fluorescent detection of DCFH-DA probe methods were studied the biological effects of Mogroside IIE on lipopolysaccharide(LPS)-stimulated RAW264.7 cell inflammation and oxidative stress.Through the comparison and analysis of this paper,People can understand the mechanism of action of Mogroside IIE to promote human health more comprehensively and objectively.We lay a theoretical foundation for the further application of Mogroside IIE in the field of food and medicine,and help us to evaluate the promotion of human health by Mogroside IIE from proteome level and cell signal transduction.The research contents and results are as following.1 ? RAW 264.7 mouse macrophages were pre-cultured for 24 hours,and lipopolysaccharide LPS stimulated RAW264.7 cells to establish a model of cellular inflammation.It was found that Mogroside IIE dose-dependently inhibited the expression of nitric oxide synthase(INOS),epoxidase synthase 2(COX-2)and prostaglandin E2(PGE2)in cells.Mogroside IIE can also significantly reduce the phosphorylation levels of p-38,MEK,JNK and I?B?.This indicates that Mogroside IIE is activated by down-regulating MAPK-NF-?B inflammatory signaling pathway.Reduced the expression of inflammatory target protein iNOS and COX-2,inhibited the production of inflammatory factor PGE2.Thus exerting its anti-inflammatory properties2?Cells in log phase were seeded in 6-well and 96-well plates for 12 hours,LPS induced establishment of cellular oxidative stress model,Fluorescence microscopy and fluorescence microplate reader to detect changes in intracellular reactive oxygen species,It was found that Mogroside IIE exhibited its antioxidant effect by scavenging intracellular ROS.3?RAW 264.7 mouse macrophages were pre-cultured at a cell concentration of 1×10 6 cells/bottle for 24 hours,and LPS stimulated RAW264.7 cells to establish a model of cellular oxidative stress.Western blot analysis of Nrf2,HO-1 and NQO1 results,Mogroside IIE is likely to activate the Nrf2/ARE signaling pathway,activation of downstream antioxidant proteins and phase II detoxification enzymes,resisting oxidative stress,exerts its biological properties against oxidative stress.Mogroside IIE also control the activation of PI3K/AKT1 signaling pathway,These results indicate that Mogroside IE may exert its oxidative stress physiological function and exert its anti-inflammatory effect by regulating PI3K/AKT1-Nrf2/ARE signaling pathway.The above results indicate Mogroside IIE can block the overproduction of ROS in cells and activate the Nrf2/ARE signaling pathway.At the same time,Mogroside IIE also regulates signaling pathways such as PI3K/AKT1 and MAPKs to simultaneously activate the Nrf2/ARE antioxidant signaling pathway and inhibit the NF-?B inflammatory signaling pathway to enhance the expression of antioxidant proteins and detoxification enzymes,and to reduce the production of inflammation-related proteins and cytokines,Thereby exerting its anti-inflammatory and anti-oxidation effects,it provides a molecular basis and theoretical basis for the development of dietary supplements and their clinical application.