Detection Of Quinolones In Fish By Graphene Quantum Dot Biomimetic Nanomaterial Biosensor

In recent years,biomimetic nanotechnology of mussels has attracted widespread attention in many popular industries and fields such as the military,industrial manufacturing,medical,and construction industries.Mussel bionic technology is a huge project.In order to better develop this technology,many new methods are constantly emerging.Based on graphene quantum dots,this paper prepares biomimetic nanocomposites of graphene quantum dots?DA-GQDs?based on mussel bionic nanotechnology,and uses them as fluorescent probes for the application of quinolones in fish.The development of biomimetic nanomaterials has laid a theoretical foundation.The main research contents are as follows:1.Based on graphene quantum dots?GQDs?,a new type of nanocomposite DA-GQDs was prepared by mussel bionic nanotechnology.The surface morphology and structure were characterized by transmission electron microscope,infrared spectrum and fluorescence spectrum.The results showed that DA-GQDs were successfully synthesized and presented as a monodispersed system with an average diameter of 60 nm,rich in functional groups,and the fluorescence intensity and position would change with changes in the excitation spectrum.Further research showed that DA-GQDs had good light stability and salt resistance,showing a good application prospect.2.A new DA-GQDs fluorescence sensor system based on mussel bionic technology was constructed for the quantitative detection of quinolone drugs,including four generations of quinolone antibacterial drugs:ciprofloxacin,moxifloxacin,and gatifloxacin star,norfloxacin.Under the best conditions,this protocol showed excellent sensitivity to these four quinolone drugs.For example,the concentration of ciprofloxacin in the range of 4×10^-8 to 2×10^-6 mol/L was good.Linear relationship with detection limit of 2.16×10^-88 mol/L.It showed that the method had low detection limit and wide linear range,and in the actual sample detection,the recovery of ciprofloxacin reaches 113%-108.7%.Therefore,this method can be used for the quantitative detection of quinolone drugs in fish with satisfactory results.3.Based on NH₂-Fe₃O₄ and DA-GQDs composite materials,a highly efficient adsorbent Fe₃O₄-DA-GQDs was constructed,and ciprofloxacin was used as the pollutant model.The adsorption test experiments?adsorption time,adsorption temperature?under different conditions were analyzed.Initial solution concentration,solution pH).The results showed that the maximum adsorption of ciprofloxacin by Fe₃O₄-DA-GQDs was 54.57 mg/g,and the equilibrium could be reached within 50minutes after the adsorption reaction occurred.Fe₃O₄-DA-GQDs were suitable for removing ciprofloxacin under neutral conditions.The optimum pH value was 7 and the maximum amount of ciprofloxacin solution was adsorbed at the initial solution concentration of 30 mg/L and the adsorption temperature at 25?,and the adsorption effect was the best.Therefore,this method can effectively adsorb ciprofloxacin in food,and the results are satisfactory.