The Side Chain Hydrophilic Modified Conjugated Polymer Sensor For The Analysis And Detection Of Metal Ions In Samples

Water-soluble conjugated polymer is a kind of conjugated system with large?-?*conjugated electron structure in main chain,and side chain modified hydrophilic functional group.Because of its remarkable fluorescence amplification effect,good water solubility and biocompatibility,it has attracted widespread attention.At present,water-soluble fluorescent polymers have been successfully applied to the detection of metal ions,biomolecules and cell imaging.This dissertation mainly focused on the application of water-soluble polythiophene derivative PT-PHO and poly?phenylene ethynylene?conjugated polymer PPE-O-PEG in metal ions detection and bioimaging.The specific research contents are as follows:1.Water-soluble polythiophene derivatives with phosphonic acids as the side chain groups were synthesized by FeCl₃ oxidation polymerization.The organic compound of every step in the synthesis was characterized by ¹H NMR and MS and the final conjugated polymer?PT-PHO?was also characterized by ¹H NMR,FTIR and GPC.We find that PT-PHO has a better selectivity to Al³⁺in hexamethylenetetramine-HCl(0.1 mol L^-1,pH=5)buffer solution.Under the optimal experimental conditions,PT-PHO has a wide linear range and a low detection limit for the detection of Al³⁺.Finally,PT-PHO was applied to the detection of Al³⁺content in traditional pasta and a satisfactory result was obtained in this part.2.We also find that PT-PHO can selectively detect Ca²⁺in NH₃-NH₄Cl(0.1 mol L^-1,pH=9)buffer solution.Under the optimal experimental conditions,PT-PHO has a wide linear range and a low detection limit for the detection of Ca²⁺.In addition,PT-PHO was also used for the specifical lysosomes imaging in cells.The experimental results show that PT-PHO can detect the concentration of Ca²⁺in lysosomes in this part.3.Based on the interaction between PPE-O-PEG and Pt?IV?,the potential application of PPE-O-PEG as Pt?IV?carrier in tumor therapy is investigated in this part.In the experiment,PPE-O-PEG and chloroplatinic acid are used to obtain the coordination compound PPE-O-PEG-Pt,which is systematically characterized.Cell experiment results show that PPE-O-PEG has good biocompatibility and nuclear targeting,and can be used as a carrier of platinum ions to transport Pt?IV?to the nucleus of tumor cells.Cytotoxicity experiments show that obvious apoptosis could be observed after PPE-O-PEG-Pt was added for 24 h.These results suggest that PPE-O-PEG will have a promising application in tumor chemotherapy and imaging.