Degradation Of Sulfamethoxazole And Changes Of Microbial Community Structure In Sediment Microbial Fuel Cells

At present,the pollution of antibiotics in sediments is becoming more and more serious,which will seriously affect human health.The purpose of this experiment was to study the variation of the antibiotic sulfamethoxazole and the changes of microbial community structure in sediment microbial fuel cells.By studying the changes of the antibiotic sulfamethoxazole in the sediment microbial fuel cell system,it was found that the sedimentary microbial fuel cell can effectively promote and improve the degradation of sulfamethoxazole in the sediment.At the 25th day of operation,the removal rate is reached.More than 80%.In the experimental groups SMX+V and SMX+with sediment microbial fuel cells,the removal rate was clearly found:surface>bottom>middle.The bottom>middle is mainly due to the fact that the bottom is closer to the anode of the sediment microbial fuel cell,which further confirms that the microbial fuel cell system has an influence radius.The surface>bottom is due to the surface of sulfamethoxazole resolved into aqueous solution,sulfamethoxazole has a reduced Kow,so that sulfamethoxazole can be easily resolved from the sediment into the aqueous solution,under natural conditions,sulfa The oxazole will gradually aggregate to the bottom,making the concentration of sulfamethoxazole at the bottom higher than the middle and upper portions.The effect of the anode placement mode of the sedimentary microbial fuel cell on the electricity production performance and the removal efficiency of sulfamethoxazole was investigated.It was found that the horizontal placement is more suitable for removing contaminants and the electricity generation performance is better than the vertical placement method.The actual engineering field has great reference and guiding significance.Under the action of the sedimentary microbial fuel cell system,the diversity of bacteria on the anode biofilm was reduced compared to the corresponding initial sediment,and also lower than the corresponding control group without the deposition of microbial fuel cells after the end of the operation.Because after 45 days of operation,the anode in the closed circuit system will enrich the electricity-producing microorganisms,thereby reducing the diversity of the bacterial community.To study the microbial community structure affected by antibiotics and sediment microbial fuel cells,high-throughput sequencing results show that in the river and lake sediment microbial fuel cells,compared with the initial experimental group CK₀,the distance from CK₀ is from near to far;NoSMX,CKno,SMXH,where SMXV and CKsmx are far away from each other.SMXV indicates that the sample is added with sulfamethoxazole and the sediment microbial fuel cell is placed vertically.CKsmx represents the sample added with sulfamethamine.The oxazole experimental group was sampled after 45 days.It can be seen that the addition of sulfamethoxazole and the vertical placement of the anode deposit microbial fuel cells can have a significant impact on the microbial community structure.Significant changes in deposits near the anode were in the CKsmx experimental group in the Burkholderiaceae family and in the SMXV experimental group in the Comamonnadaceae family.