Design And Performance Of Fluorescence Chemical Sensor For The Identification And Detection Iron Ions

As a kind of fluorescent chemical device,fluorescent chemical sensor has the advantages of simple operation,low cost,high sensitivity,selectively identity metal ions.Iron is an important microelement and its content in the environment and human body must be kept in balance and stable.Therefore,it is of great value in practical application to design fluorescent chemical sensors that can monitor Fe³⁺ content in environmental and life systems.In this paper,three novel small-molecule fluorescent chemical sensors for identifying Fe²⁺and Fe³⁺ were designed and synthesized,and their performance of recognition and application were investigated.The main contents of the paper:1.Sensor 1 was synthesized by 2-picolinic acid and oxadiazole,and its structure was characterized by 1H NMR,13 C NMR and MS,respectively.Fluorescence spectra tests showed that sensor 1 displayed satisfactory fluorescence recognition performance for Fe²⁺ and Fe³⁺ in mixed solution of THF and water.IR,mass spectrometry and DFT theoretical calculation proved that sensor 1 formed a 1:1 non-fluorescent complex with Fe²⁺ or Fe³⁺.The experiments demonstrated that sensor 1 had excellent application value to detect Fe²⁺ and Fe³⁺ in actual samples and living cells.2.Two water-soluble fluorescent chemical sensors?sensor 2 and sensor 3?were synthesized by benzothiazole and salicylic acid,and their structures were characterized by 1H NMR,13 C NMR and MS,respectively.Fluorescence spectra tests demonstrated that two sensors presented excellent fluorescence recognition performance for Fe³⁺.IR and titration experiments proved the non-fluorescent complexes between sensors and Fe³⁺ had 1:1stoichiometry ratio.Experimental results showed that sensor 2 and sensor 3 displayed low cell toxicity and could be used to detect Fe³⁺ in living cells.