Study On The Enrichment Method Of Catecholamine And Sulfonamide On High Performance Capillary Electrophoresis

High performance capillary electrophoresis?HPCE?is a new type of liquid phase separation technology that allows components to be measured to pass through capillary separation channels under a high-voltage electric field.It is used in many fields in modern analysis..High performance capillary electrophoresis technology has the advantages of high performance,separation of samples in a short time and multiple separation modes.The detection of catecholamine content is of great significance,because catecholamines are important neurotransmitters in the human body,its content is closely related to mental illness.Sulfonamides are antibacterial drugs,which are widely used in modern production and life.However,its difficult to metabolize properties will cause residues in animals and affect human health and ecological environment.In this article,the on-column enrichment technology of capillary electrophoresis for the separation of sulfonamides and catecholamines are we studied including the field enhanced sample injection-micellar solvent stacking capillary zone electrophoresis technique and micelle cyclodextrin stacking.The details are as follows: Chapter 1: IntroductionThis chapter introduces the review of capillary electrophoresis,and the classification and research progress of capillary enrichment techniques in capillary electrophoresis.The significance of this article.Chapter 2: Field enhanced samples injection-micellar solvent stacking capillary zone electrophoresis technique for determination of catecholaminesIn this chapter,dopamine,norepinephrine and epinephrine are measured by capillary electrophoresis.Field amplification-micellar solvent accumulation capillary enrichment technology was used in combination to reduce the detection limit of catecholamines Under the optimal conditions,the detection limits of the catecholamine components were 5.64 × 10^-9 mol / L,6.80 × 10^-9 mol / L,and 4.73 × 10^-9 mol / L,respectively.This method has been successfully used for the determination of catecholamine content in toad venom raw materials.The operation procedure is simple and the detection is highly efficient and sensitive.Chapter 3: Micellar to cyclodextrin stacking and micellar to organic solvent stacking detection of sulfonamides respectivelyThis chapter uses micelle to cyclodextrin stacking and micelle to organic solvent stacking to detect five sulfonamide drugs.Under the reverse voltage,the solution of the test component containing micelles interacts with ?-cyclodextrin and methanol,the organic solvent,to release the test component to the micelle solution,and to reverse the direction of electrophoresis of the test component.Under negative pressure,the sample solution containing micellar interacts with ?-cyclodextrin and methanol,respectively,to release the components to be tested in the micellar solution,and to reverse the electrophoretic direction of the components to be tested to enrich the five sulfonamides.Micellar to cyclodextrin stacking method to enrich the sulfonamides best experimental conditions: 100 mmol/L sodium dodecyl sulfate,phosphoric 100 mmol/L acid,12% acetonitrile,30 mmol/L cyclodextrin,The injection time ratio of cyclodextrin is 1: 2,and the separation voltage is-16 k V.The best experimental conditions for the enrichment of sulfonamides by the micelle to methanol stacking method: 100 mmol/L sodium dodecyl sulfate,50 mmol/L phosphoric acid,15% acetonitrile,30% methanol,injection time ratio 1: 1.Separation voltage was-16 kV.