Physico-chemical Property And Antibacterial Application Of The Gelatin From Swim Bladder Of Sturgeon

As a kind of modern fish processing waste,Sturgeon swim bladder contains a lot of collagen.It has long been favored by China and Southeast Asian countries as a food and medicinal material and tonic.However,with the increasing product of sturgeon,a large amount of sturgeon swim bladder has not been scientifically applied.In this study,sturgeon swim bladder was used as a raw material to establish an efficient extracting technology of gelatin without acid and alkali pretreatment,and to clarify the physico-chemical properties of gelatin.The nanofiber membrane of gelatin/galangal essential oil/?-cyclodextrin?SG/GRO/?-CD-IC?was successfully prepared by electrospinning technology,and its physico-chemical and antibacterial properties were analyzed.The main contents and results are summarized as follows:?1?Under the optimal extraction conditions of 50?,30 min and 10 mL/g,the yield of gelatin was as high as 94.15%?w/w?,and it showed similar amino acid profile and electrophoresis pattern to type I collagen.Compared to commercial porcine?PG?,bovine?BG?and fish skin gelatin?FG?,it exhibited higher molecular size?9.84 kDa?,emulsion activity?171.76 m²/g?,gel strength?853.23 g?,water-holding capacity?92.37%?,viscoelasticity?tan?,0.03?and whiteness??E,3.38?.But the transmittance(T_{450 nm}=40.56%,T_{620 nm}=59.07%),emulsion stability?30.09 min?,foam expansion?203.00?and stability?26.92?,gelling?16.88??and melting temperature?21.80??were lower.While the pH?6.87?and viscosity?28.60 mPa·s?were moderate.In addition,the gelatin could be made into high-quality hydrogels and electrospinning nanofibers.?2?The main components of GRO were analyzed by GC-MS,including Ethyl cinnamate?37.56%?,Methyl cinnamate?19.99%?,n-Pentadecane?14.61%?,Cineole?9.06%?,3-?-Carene?3.46%?,?-?-camphene hydrate?3.24%?,Isobornyl acetate?2.78%?,18-Crown 6-Ether?2.48%?,L?-?-Borneol?0.67%?.The SG/GRO/?-CD-IC nanofiber membrane was successfully prepared by electrospinning technology,which showed good thickness?0.13 mm?,water solubility?5.06%?,contact angle?89.55°?,water vapor transmission rate?0.49 g·mm/?m²·h·kPa??and controlled release capability?7 d,64.87%?.SEM and FTIR results showed that GRO was included in?-CD,the GRO composition after inclusion was not significantly changed,and the inclusion compound?GRO/?-CD-IC?could be bound to nanofibers.SG/GRO/?-CD-IC nanofiber membrane could destroy the cell wall of Escherichia coli?O157:H7?,caused cell melting and ulceration,and showed significant antibacterial effect.