Fabrication Of Gel Electrophoresis Device By Using 3d Printing

Compared with traditional manufacturing,3D printing technology provides a convenient and reliable method for making complex systems quickly,accurately,and at low cost.Due to the great advantages of this technology,it has evolved rapidly in recent decades and has been applied in many fields,such as the manufacture of aerospace aircraft,flexible robots,and medical equipment.In the robot manufacturing industry,although rigid robots under traditional manufacturing technology have high accuracy,they cannot be applied to some fields that require complex structures.3D printed flexible robots have the ability to deform elastically and can be manipulated in narrow spaces.Therefore,this paper uses 3D printing to improve the design of the gel electrophoresis system for the gel electrophoresis system,build a protein separation system,and complete the design of the gel electrophoresis system.In recent years,continuous flow gel electrophoresis combined with inductively coupled plasma mass spectrometry(GE-ICP-MS)has been successfully applied to the separation and determination of metallo-proteins.However,the continuous flow gel electrophoresis system currently used belongs to the preliminary design and processing of this laboratory,and there are still many imperfections in design and processing.Based on this,this research uses 3D printing technology to design and develop two types of gel electrophoresis devices for the separation and determination of proteins and metallo-proteins according to different usage scenarios.Firstly,we applied 3D printing technology to develop a portable gel electrophoresis device for rapid screening of protein samples.In this work,we designed a portable gel electrophoresis device for rapid protein separation and detection.The size of this portable gel electrophoresis tank is only 15 × 20 × 17 mm.A total of 4 mL of electrophoresis buffers were used for the positive and negative electrodes.A 25 V constant-voltage lithium battery can support the continuous operation of the electrophoresis system for about 100 hours.Gel electrophoresis devices can be conveniently,quickly processed and manufactured at low cost with 3D printing in the lab.By comparing the separation effect of this device on commercial color protein standards,we have repeatedly optimized its structure.The electrophoresis device was then used to separate five commonly used standard proteins,and the results were compared with those of commercial plate gel electrophoresis.The results show that the portable electrophoresis device can quickly and efficiently separate proteins.The continuous development of 3D printing technology will greatly promote the development and development of miniaturized and portable experimental equipment.Secondly,we applied 3D printing technology to develop a gel electrophoresis device for the separation and detection of metal-binding proteins.The device includes an elution system,a cooling system,an electrophoresis system,and an integrated housing.The elution system uses the construction of a dual injection pump platform to communicate with a computer terminal using the DT protocol to achieve advection mode.The cooling system uses the principle of semiconductor refrigeration.The STC15F2K60S2 single-chip microcomputer is used as the core processor of the entire system.It adopts a modular design to control the temperature adjustment of the cooling system.The electrophoresis system uses the principle of gel electrophoresis to design and manufacture a horizontal columnar gel electrophoresis device and an electrophoresis power supply for protein separation.The gel electrophoresis device in this study can be combined with inductively coupled plasma mass spectrometry(ICP-MS)to form a continuous flow GE-ICP-MS system for online separation and detection of metalloproteins.In this study,the gel electrophoresis system was designed systematically and assembled and operated in the laboratory.In this study,customized modifications and optimizations based on digital prototypes are easy to achieve,highlighting the applicability of 3D printing to facilitate the development of analytical devices.