Screening And Promoting Effect On The Growth Of Two 1-aminocyclopropane-l-carboxylic Acid (ACC) Deaminase-containing Plant Growth-promoting Rhizobacterias (PGPR) From Rubber Tree

Tapping panel dryness is a kind of natural rubber production of secant partial or all don’t glue symptoms,has become one of the main factors that restricted natural rubber production.There are many TPD hypothesis at home and abroad,the mechanism of which Cai Lei etc.put forward the "ethylene induced TPD" which is more and more recognition with ethrel stimulation tapping the implementation of new cutting system.One of PGPR with ACC deaminase activity and from rhizosphere bacteria(Plant growth promoting rhizobacteria,PGPR)can use the ACC(ethylene precursor)as a nitrogen source to reduce ethylene content,stimulate plant growth and development.The aim of this study is from the rubber tree rhizosphere filter can be applied to rubber strain of prevention and control of dead skin,and carries on the thorough research,in order to be able to develop a prevention and control of rubber tree dead skin biological bacterium agent or extraction of the active substance,died for rubber skin drugs for prevention and control and the application to provide a new way.1.From rubber gardens mainly adopt 18 soil samples which were isolated with ACC deaminase activity the rhizosphere bacteria of 152 strains,the ACC deaminase activity range is 1.27*10-4 U/mg-0.226 U/mg,two bacterial strains selection activity is higher(HBRM-86 and HBRM-16,ACC deaminase activity were 0.226 U/mg and 0.164 U/mg)to carry out the follow-up study.By bacteria morphology observation,physiological and biochemical experiments and 16SrDNA molecular biology identification,will eventually to be certainly HBRM-86 and HBRM-16 Enterobacteraerogenes and Serratiamarcescens.Two strains of bacteria to produce capability,the IAA were 19.007 ug/mL and 3.883 ug/mL,significantly higher than that of control;Strain HBRM-16 chelate ring diameter(D)and colony diameter(d)ratio was 2.90;Inorganic phosphorus in the medium,HBRM-86 soluble phosphorus ability stronger than the HBRM-16,in the use of of organophosphorus,HBRM-86 is not dissolved phosphorus ability,HBRM-16 in dissolved organic phosphorus ability.In short,the ACC deaminase activity high strain HBRM-86 and from two potential index is better than HBRM-16,but HBRM-16 the growth potential of comprehensive.2.Through potted plant experiment inquiry HBRM-86 and HBRM-16 the promoting effect of echo application prospect.In the measurement of plant biomass,the seedlings of rubber plant height,stem diameter,fresh weight and dry weight were determined and compared,the results biomass of treatment group is significantly higher than CK group,height above ground in T1W is 1.04 times that of CKW,T2W is 1.08 times that of CKW;Dry and fresh weight of T2M underground part of the fresh weight is 1.12 times that of CKM,T2M underground part of the dry weight is 1.16 times of CKM.That means with ACC deaminase activity strains HBRM-86 and HBRM-16 of rubber seedling biomass has a certain role in the growth,and in this process,the strain HBRM-16 effect is better;In strain measurement of plant photosynthesis,including seedling leaf photosynthetic characteristics(net photosynthesis,transpiration rate,stomatal conductance and intercellular CO2 concentration and chlorophyll content,chlorophyll fluorescence kinetics parameters),is one of the most significant T2W group of chlorophyll a(Ca)and chlorophyll b(Cb)content were significantly higher than that of CKW group(P<0.05,respectively 1.16 times and 1.16 times that of CKW,while the other two indicators of the result is not significant,but all illustrates the drench bacteria liquid for rubber seedlings have promote role;In terms of strain on the influence of plant resistance,measured the contents of soluble protein content,MDA,GSH,H2O2 content,SOD and POD enzyme activity index.Results show that though at different levels of each target grows,but water bacteria liquid has promoting effect on the resistance of the rubber seedling is,the result is especially apparent in the H2O2 and POD.3.With GT1 as the research object,explore different concentrations HBRM-86 and HBRM-16 bacteria liquid on the effect of the west to find its optimum concentration,ease of production and application.On the ground of the west part of the height,stem diameter,fresh weight,dry weight and root system related indexes were determined,it can be seen that bacteria liquid of lower after the first increases with the decreasing of concentration,near the handle 1 has certain inhibitory effect,in the treatment group 3,4,5,when the peak,it shows that strain germination and growth has a promoting effect of the west,and the best concentration within 10-2～10-4.