Expression And Funcyional Analysisof A Wheat Hexose Transporter Gene (Ta STP13) Induced By Pucciniastriiformis F.sp.tritici

Wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,is considered one of the major wheat fungal diseases.Wheat stripe rust is an obligate parasitic fungi living nutrition,must absorb sugar and other nutrients for their growth and development through the suction structure infection from host cells.Due to Puccinia striiformis f.sp.tritici breeding,propagation,mutation,toxicity and it is not artificially controlled very quickly,so the use of general control methods and measures may be not enough.Thus,For wheat-stripe rust prevention and control work,can start from the molecular mechanisms of absorption in the wheat hexose and develop their practical and effective strategies for the prevention and treatment.1.This study has been constructed from Wheat-stripe rust interaction cDNA library screening to obtain wheat hexose transporter gene TaSTP13 by bioinformatic analysis.Through whole-genome sequence alignment TaSTP13 found in 4A,4B,4D chromosomes have homologous sequences,we cloned the full length of TaSTP13,sequence length 1545 bp.Analysis of amino acid sequence encoded TaSTP13 showed that the gene encodes a 514 amino acids,molecular weight 56.7kDa,protein isoelectric point(pI)of 8.95;TaSTP13 gene domain analysis,domain containing sugar transporter,which contains l2 transmembrane domains;2.Phylogenetic analysis showed that the genetic relationship of TaSTP13 and At STP13nearer;Promoter sequence analysis showed that the selected area contains the core promoter and response elements upstream,cis-acting elements present in the region have ABRE,Box-W1,ERE,etc.,they involved in the regulation of plant stress;3.By injection tobacco transient expression of fusion proteins,the study found TaSTP13 mainly distributed in the cell membrane;4.Yeast hexose transporter mutant expression TaSTP13,which TaSTP13 have capable of transporting glucose,fructose,mannose,galactose,indicating TaSTP13 encoding hexose transporter gene;5.TaSTP13 homologous sequences on chromosome 4A,4B,4D expression analysis by qRT-PCR technology respectively,found TaSTP13 present in all tissues,the highest expression in leaves,stems and flowers of expression is relatively low;under the four abiotic stresstreatment,TaSTP13 relative expression levels are up-regulated in the state in which the wound and low temperature relative expression levels more than 10 times,drought relative expression about 5 times,salt relative expression about 2.8 times;under the four plant regulators,TaSTP13 relative expression levels of performance are up-regulated in a high state,the highest expression of ABA,ETH,MeJA,SA treatment in more than 40 times;the level of the TaSTP13 transcription in incompatible and compatible interaction is expressed,and the expression is relatively high in the compatible interaction.6.To obtain direct evidence for the function of TaSTP13 during the wheat-Pst interaction,silencing efficiency test requirements,silence TaSTP13 stripe rust mycelium growth retardation,developmental hyphae abnormal phenomenon enlargement,and reactive oxygen species associated genes down-regulated,up-regulated the expression of defense-related protein gene.These results suggest that: TaSTP13 induced by biotic stress,abiotic stress and hormone treatment,and to participate in the stress response,the exercise of their functions.TaSTP13 in wheat-stripe rust interaction system,may directly or indirectly affect regulation of stripe rust mycelium expanded by hexose transport and thus participate in the susceptible process.