The Mechanism On Amino Acid Of Black Tea With Red Light Withering Based On The Technology Of RNA-seq

Black tea is one of the six major tea,wilting has important influence on the quality of black tea.Other studies have shown that the quality of the tea can be improved by light wilting,and lights can affect the contents of sugars,amino acids,polyphenols,flavonoids and other ingredients in the plants respectively,and these ingredients are the main quality components in black tea.According to the physicochemical analysis,the content of amino acids,theaflavins,catechins of wilting leaves processed by red light is higher than other light quality processing,amino acids particularly.Therefore,in this paper,withred and green wilting,compared by natural withering,it analyzes the physical and chemical quality,and with the help of transcriptome sequencing technology,it explores the mechanism of effect on amino acid of tea leaves with red light wilting.The results are as follows.1.Different light quality with the same wilting timeThe content of amino acid,catechin,tea polyphenols,soluble sugar and caffeine processed by red light and natural wilting has no significant difference,while the content of chemical compositions of green light withering is significantly lower than that of red light and natural withering.2.Same light withering with different wilting timesDuring withering process,the total amino acid,catechin,tea polyphenols,soluble sugar of withering leaves has decreased after rised first,and caffeine content has been increasing gradually.Besides,the changes of chemical compositions of red light withering are more significant than that of green light or natural withering,and red light has a greater influence on the physical and chemical compositions for the withering leaves,and it improves the quality of black tea.3.The amino acid metabolism and its related substance of withering with different times and lights(1)In early period of red light and natural withering,processed by same light with different times,the enzyme regulating the synthesis of aspartic acid,glutamine aminotransferase,is increasing expressed,and the enzymes regulating the decomposition and transformation of aspartic acid,aspartate aminotransferase and aspartate oxidase,are decreased expressed,and the enzyme regulating the synthesis of glutamic acid,glutamate synthase,is up-regulated expressed,and the enzyme regulating the decomposition of glutamic acid,glutamine synthetase),is down-regulated expressed,and the enzymes regulating the synthesis of theanine precursor substances,GOG AT、GDH and BAPAT,are up-regulated expressed,thus L-arginine,glutamic acid and theanine have been accumulated.In the late period of withering,L-aspartate,theanine decreased significantly,which may due to the higher decomposition rate.Besides,few precursor substances are generated which reduces the rate of synthesis.(2)In the early period of withering,the expressions of phosphoenolpyruvate carboxykinase,pyruvate decarboxylase and malate dehydrogenase of red light withering are significantly lower than that of natural withering which leads to lower content of L-aspartic acid of red light withering than that of natural withering in the late period.Despite the expression of Isocitrate dehydrogenase of red withering is significantly lower than that of natural withering,the content of glutamic acid of red light withering is significantly higher than that of the natural processing in the late period.And the reasons may be that the decomposition and transformation rate of glutamic acid processed by red light is lower than that processed by natural process,which results in accumulation of glutamic acid of red light withering.