| Tilapia streptococcus disease is a infectious diseases,it will cause a series of physiological and pathological changes and to death.Because of the diseases come on quickly,contagion is strong,mortality rate is high,Lead to a pandemic outbreak easily.The diseases bring huge economic losses to the world of aquaculture industry.Guangdong province is the leading producer in China.The report of tilapia streptococcus disease showed a trend of increase in recent years,so it is need to study the means of prevention and control tilapia streptococcus disease.At present the means of prevention and control tilapia streptococcus disease have chemical prevention and control,ecological prevention and control,prevention and control of the immune.Ecological prevention and control can decrease outbreak rate of tilapia streptococcus disease,have greatly influenced in the water quality and inhibit the growth of the fish.The study on prevention and control of the immune are more,There are many different kinds of vaccines,update soon,but the vaccines easy deactivation,operation trival.Chemical prevention and control use antibiotics,efficacy work fast and strong,but it easy to produce tolerance and drug residues.Modern pharmacological studies have shown that traditional Chinese medicine of clearing away heat have clearly antibacterial activity,Many traditional Chinese medicine and compound have broad spectrum of antimicrobial activity,to restore the human body micro ecological balance,regulation of the immune,increase anti-infection,.These advantages can make traditional Chinese medicine solve bacteria drug resistance questions.So traditional Chinese medicine prevention and control tilapia streptococcus disease have very practical significance.ObjectiveTilapia streptococcus disease broke out and popular,identification of tilapia streptococcus strain,many traditional Chinese medicine extract for inside and outside the body pharmacodynamics experiment,looking for traditional Chinese medicine active ingredients,provide the experiment evidence for the new product development.MethodsThis subject go through identify the bacterial species,Screening high sensitive traditional Chinese medicine(TCM)extract from 23 kinds TCM aqueous extract and alcohol extract through the drug sensitive test.The next research is pharmacodynamics experiments on animals,it is reflect the antibacterial,bactericidal,antioxidative ability from mortality rates and indicators of LSZ,CAT,T-T-SOD,NO,MDA,GOT,GPT results.And then confirm Curative effect of the extract.1.Identification of bacterial species:Through the separation and purification of bacterial species,using microscopic identification,biochemistry identification and PCR identification to identify the bacterial species.2.Drug sensitive test:using paper method,Select high sensitive traditional Chinese medicine(TCM)extract from 23 kinds TCM aqueous extract and alcohol extract,for Determination of the MIC and MBC.3.Pathogenicity test:By the pathogenicity test to determine the virulence,intraperitoneal injection dose of streptococcus agalactiae of tilapia by 0.05mL/10g for model.To observe 7d and determination of mortality rates,to observe infection situation about the abdominal cavity and internal organs,sampling from internal organs for in vitro culture.To determine the best model concentration.4.Pharmacodynamics experiment:Drug normal tilapia by 2.5g/Kg/d.continuous for 21d.In the 15d,using the best model concentration intraperitoneal injection dose of streptococcus agalactiae of tilapia by 0.05mL/10g into drug group and model group.statistics the deaths rate in 15-21d,take the liver and serum for LSZ,CAT,T-SOD,NO,MDA,GOT,GPT results.Results1.Out of body experiment:Through experiment identified this bacterial species was Streptococcus agalactiae of tilapia.Screened out Calla Chinensis,Herba Rabdosiae Serrae,Radix Sophorae Flavescentis extract what is high sensitive traditional Chinese medicine(TCM)test by Drug sensitive test.2.Experiments on animals:The model dose was 2.58 × 105CFU/mL.Herba Rabdosiae Serrae group,Radix Sophorae Flavescentis group can decreased the mortality rates and increased the content of blood serum lysozyme,Calla Chinensis group can decreased the content of blood serum lysozyme;Herba Rabdosiae Serrae group,Radix Sophorae Flavescentis group and Calla Chinensis group can decreased the content of NO in liver tissue;Herba Rabdosiae Serrae group,Radix Sophorae Flavescentis group can decreased the content of MDA in liver tissue;Herba Rabdosiae Serrae group and Calla Chinensis group can increased the content of CAT in liver tissue;Radix Sophorae Flavescentis group had decreased the content of CAT in liver tissue tendency;Herba Rabdosiae Serrae group,Radix Sophorae Flavescentis group and Calla Chinensis group can increased the content of T-SOD in liver tissue;Herba Rabdosiae Serrae group,Radix Sophorae Flavescentis group and Calla Chinensis group can increased the content of GPT in liver tissue and decreased the content of GOT in liver tissue.The above result are have Statistical significance.ConclusionHerba Rabdosiae Serrae group,Radix Sophorae Flavescentis group can decreased the mortality rates.Herba Rabdosiae Serrae group went through increased the content of LSZ,CAT and decreased the content of MDA,NO,GOT to get the bactericidal,antioxidative ability;Radix Sophorae Flavescentis group went through increased the content of LSZ,T-SOD and decreased the content of MDA,NO,GOT to get the bactericidal,antioxidative ability;Calla Chinensis group went through increased the content of CAT,T-SOD and decreased the content of MDA,NO,GOT to get the bactericidal,antioxidative ability. |
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