Research Of My Cotoxin Chemotvpe And Pathogenecity In Wheat Of Fusarium Graminearum In Huanghuai Regions

Fusarium graminearum is the main pathogen of Fusarium Head Blight,Fusarium Crown Rot,Maize Ear Rot and Maize Stalk Rot.In recent years the diseases above aggravate year by year because of the implementation of straw returning field generally and the climate warming.It has become the important factor on restricting the yield and quality of wheat and maize.Previous studies have indicated that F.graminearum can produce some toxins such as DON and NIV and so on and the mycotoxin chemotype in different regions has large differences.Otherwise there are also differences on pathogenicity for different toxin producer in F.graminearum.To identify the relation between mycotoxin chemotype and pathogenicity in F.graminearum of wheat and maize in Huanghuai areas.In 2014 and 2015 infected wheat and maize are widely sampled in the main areas of wheat and maize production in Huanghuai areas.Through morphological identification and molecular biological identification F.graminearum is identified.Then detect the types of mycotoxin and identify the pathogenecity to the head and stem of wheat.Analyze the relationship between mycotoxin chemotype and pathogenecity.The main results are as follows.Through separating the pathogen from wheat and maize a total of 252 F.graminearum isolates are identified by using specific primer Fg16F/Fg16 R.109 F.graminearum are separated from the disease heads of Fusarium Head Blight,and 48 F.graminearumare separated from the diseased stems of Fusarium Crown Rot,and 33 F.graminearum are separated from the diseased ears of Maize Ear Rot,and 62 F.graminearum are separated from the diseased stems of Maize Stalk Rot.The mycotoxin chemotype is detected on 252 F.graminearum by using the primers Tri303F/Tri303 R and Tri315F/Tri315 R.181 F.graminearum isolates are the 15-Ac DON chemotype(71.83 %).65 F.graminearum isolates are 3-Ac DON chemotype(25.79 %).While only 6 F.graminearum isolate is the NIV chemotype(2.38%).It suggests that the 15-Ac DON producer is the dominant population,and the 3-Ac DON producer comes second,and the proportion of the NIV producer is low.In Hebei areas all of the isolates produce 15-Ac DON mycotoxin.In Henan areas all of the 3 mycotoxin producers exists,and the main mycotoxin chemotype is 15-Ac DON.In Anhui and Jiangsu areas all of the 3 mycotoxin producers exist,but the main mycotoxin is 3-Ac DON.The pathogenicity detection to the heads of wheat shows a significant differences between 77 F.graminearum.In aikang 58 only 1 F.graminearum belong to weak virulent strain,and 33 F.graminearum belong to medium virulent strains,and 43 F.graminearum belong to strong virulent strains.In zhoumai 27 4 F.graminearum belong to weak virulent strain,and 50 F.graminearum belong to medium virulent strains,and 23 F.graminearum belong to strong virulent strains.Through analyzing the pathogenicity of F.graminearum of different mycotoxin chemotype,we can find that the in aikang 58 the pathogenicity of 3-Ac DON producer is strong,and there is no obvious pathogenicity difference between 15-Ac DON and NIV producer.In zhoumai 26 the pathogenicity of NIV producer is weak,and there is no obvious pathogenicity difference between 15-Ac DON and 3-Ac DON producer.The pathogenicity detection to the stems of wheat shows a significant differences between 78 F.graminearum.In aikang 58 2 F.graminearum belong to weak virulent strain,and 12 F.graminearum belong to medium virulent strains,and 64 F.graminearum belong to strong virulent strains.In zhoumai 26 3 F.graminearum belong to weak virulent strain,and 19 F.graminearum belong to medium virulent strains,and 56 F.graminearum belong to strong virulent strains.Through analyzing the pathogenicity of F.graminearum of different mycotoxin chemotype,we can find that in aikang 58 the pathogenicity of 3-Ac DON and NIV producer is stronger than the 15-Ac DON producer.In zhoumai 26 the pathogenicity of NIV producer is strong,and there is no obvious pathogenicity difference between 15-Ac DON and 3-Ac DON producer.The pathogenicity results to heads and stems of wheat of F.graminearum indicate that there is obvious difference in different strains.Most of them are strong and medium virulent strains,and there is no obvious correlation between pathogenicity and mycotoxin chemotype of F.graminearum.