Physiological Response Of Leymus Chinesis With Different Leaf Colors On Drought And Salinity Stress

The cline is selecting the continuous variation of the pressure in the geographical space,which is resulting in gene frequency and phenotype of the gradient,the formation of a gradient of variation groups.Leymus chinensis is the dominant species in the northern grassland,which is adaptability and wide distribution,the majority of the area of distribution in China's northern grasslands.The habitat of distribution are differences,the formation of the different phenotypes of L.chinensis populations,one of the most obvious phenotype is the leaf color differences.L.chinensis leaves cline between the different soil types in different geographical region or geographical area,but the leaves of the phenotypic changes accompanied by drought salt tolerance alkali physiological differences is unclear.Therefore,this study as the research object the L.chinensis leaves cline to the Changling,Guyuan and Tumuji different areas of different soil types,to study the simulated drought and salinity conditions the Leymus chinensis cline in salinity and drought stress under internal physiological response mechanisms as well as differences in study reveals the L.chinensis leaves cline drought salinity physiological differentiation and variation law,deepen the theoretical basis of population ecology,but also to improve the productivity of Leymus chinensis steppe,protection forage resources provides a theoretical basis for the operation and management of grassland science.Drought stress physiological response to the test results show that osmotic adjustment five leaves of L.chinensis(the gray-green,the hypo-gray-green,the transition color,the hypo-yellow-green and the yellow-green)in leaf water content,leakage rate,most of the MDA and soluble protein reached a significant or very significant correlation,and comprehensive analysis of five leaves sheepthe grass these indicators b values shows that glaucum L.chinensis were less than the yellow-green of L.chinensis glaucum L.chinensis drought is strong;chlorophyll content with drought stress enhancement,it indicates that the five kinds of divergent types of sheepgrass have a strong drought resistance,compare the b value results show that the gray-green of L.chinensis large values of b,drought photosynthetic ability to regulate;the seven antioxidant enzymes SOD,POD,CAT,GR,APX,DHAR and MDHAR,respectively,carriedanalysis,the comprehensive results showed that the gray-green of L.chinensis the overall activity of the antioxidant enzyme system slightly stronger than the other leaves of L.chinensis.Combining these results,the regulation of the osmotic adjustment and antioxidant enzyme system is an important physiological responses to adapt to the ecological environment,in drought for five leaves of L.chinensis,gray-green L.chinensis slight advantage,and different leaves of L.chinensi the drought resistance mechanisms also exist differences,five kinds of divergent types of Leymus chinensis on drought Tolerance differentiation.Salinity stress physiological response to the test results showed that under salinity stress,most of the different parts of the three color of leaves(the gray-green,the transition color and the yellow-green)of L.chinensis MDA and soluble protein were reached a significant or very significant correlation and comprehensive analysis of the gray-green of L.chinensis MDA indicators b value were showed that glaucum L,chinensis were less than the yellow-green chinensis,glaucum L.chinensis saline-alkali strong analysis of protein indicators for different regions and soil Leymus chinensis leaf gradient of the group significant differentiation,color transition of L.chinensis most saline-alkali capacity;chlorophyll content increased with salinity stress intensity,indicating that the three leaves of L.chinensis have a strong ability to saline-alkali,comparing b values results show that different parts of the soil Leymus chinensis leaves cline has incurred significant differentiation,gray-green L.chinensis large values of b,saline-alkali photosynthetic ability to regulate;the seven kinds antioxidant enzymes SOD,POD,CAT,GR,APX,DHAR and MDHAR were theanalysis,results show that the overall activity of the antioxidant enzyme system of the gray-green of L.chinensis slightly stronger than the other leaves of L.chinensis.Combining these results,MDA,protein,chlorophyll content and antioxidant enzyme system regulation is an important physiological responses to adapt to the ecological environment,three different soil types for different parts of the leaves of L.chinensis in salinity,gray-green sheep the sketch dominant,and different leaves of L.chinensis salinity mechanisms also exist differences,three divergent types of Leymus chinensis salinity physical differentiation.The test results of natural Leymus chinensis cline physiological indicators and indicators were showed that different parts of the soil organic matter content in the order was the Changling<Tumuji<Guyuan;the soil moisture order was Changling<Tumuji<Guyuan;soil conductivitythe order was Tumuji<Guyuan<Chang ling;These results suggest that low the the Changling soil organic matter content,water content,soil salt content was high,so the worst soil.Three soil bulk density and pH values gap was not large,it indicated that the three high and low soil fertility were very similar.Compare different leaf physiological indicators of the same soil in the same region,it indicated that the gray-green of L.chinensis chlorophyll index and to osmotic adjustment indicator than the yellow-green of L.chinensis,was gray-green L.chinensis adaptable,yellow-green chinensis weak,Leymus chinensis leaves cline combined with the same soil in the same areas the physiological indices and chlorophyll index correlation analysis,show that various regions of L.chinensis leaves cline soluble sugar and proline the two blades physiological indicators,respectively,and leaf green a chlorophyll b and chlorophyll a + b is significant or very significant positive or negative correlation.The results show that different regions and different soil types has a certain influence on the formation of L.chinensis cline.These results are for future study presented strong evidence that L.chinensis stress environment under physiological adaptation mechanisms and evolutionary aspects of physiological adaptation.