The Efffcts Of Allicin And Alizarim On The Trichomonas Gallinae In Vitro And Preliminary Application

Pigeon trichomoniasis is a common parasitic disease on pigeon,which is a multiple infectious protozoal disease caused by Trichomonas gallinaes.Sick pigeons usually presents gastrointestinal disease,its main feature isthe necrotic of ulcers in oral,craw,esophagus and other parts,digestive and respiratory obstruction cause prejudice to eating and normal breathing what cause dove to death.With the increase in consumption of meat pigeon industry,the number of pigeon is rising year by year,which makes the pigeon industry has been a huge growth.However,due to the unreasonable way of feeding,breeding technology deficiencies and other factors,the flock of morbidity of Trichomonas and mortality of youngsters showing an upward trend.Therefore,the use of anti-pigeon Trichomonas drug is more frequently.Currently,the chemical drugs of common treatment of pigeon trichomoniasis have tinidazole,metronidazole and other clinical,although metronidazole has a very significant effect to kill Trichomonas,But resistance of Trichomonas gallinae has a significant increase,but also the cure rate is gradually decreasing and recurrence rate has some improvement.On the other hand,not only have a greater side effects because of long-term use of metronidazole,but also has the potential danger to cause carcinogenic and gene mutations.Although some medicine works faster than herbal,but because herbal are natural and have small side effects,and therefore,more and more people favor herbal.Based on the above analysis,this study intends to study effects of extracts of herbal on the Trichomonas gallinae in vitro to provide a scientific basis for extract of garlic and madderagainst pigeon Trichomonas.Experiment I The effects observation of Allicin and Alizarin on Trichomonas gallinae Observe and explore the morphological changes of allicin and alizarin on Trichomonas gallinae in vitro culture.Methods To make hepar-peptone-glucose medium of the cultuer of Trichomonas gallinae.Collect and culture Trichomonas gallinae.After cutuerd for 24h,to purify Trichomonas gallinae and make its concentrations greater than 1 ×105/ml.Adding Allicin and Alizarin respectively which concentration is lmg/ml?1.5mg/ml to culture medium of trichomonas gallinae.cultured under 37??Then observe the morphological changes of parasites which are collected in 0.5h?2h?4h under optical microscope.Also to prepare samples of scanning microscope at different times,record results and analysis.Results The vitality of Trichomonas gallinae in drug group started to decline in 0.5h under optical microscope,flagella attached to parasites in 2h,Vacuoles appeared in the internal of parasites in 4h,then parasites died.When scanning electron microscope observed,the surface of Trichomonas gallinae becomes uneven in 0.5h.As the time increases,sunken appeared in 2h and flagella attached to parasites.surface depression of parasites started to deepen,parasites began to lysis and contents leaked,then Trichomonas gallinae died.Experiment II Allicin's pharmacokinetics determination in animals This experimental study Allicin's pharmacokinetics determination in animals.Methods After the solution of allicin administered orally,with HPLC determine Allicin's pharmacokinetics determination in animals in 24h.Results Allicin through into the blood of pigeon with oral just measured the date in0.08h?0.25h?0.5h.Experiment ? The effects of the extracts of Allicin and Alizarin on the Trichomonas gallinae in vitro To study the effects of Allicin and Alizarin on the Trichomonas gallinae in vitro and the preliminary application.Methods Trichomonas vaginalis was treated with different concentrations of Allicin?Alizarin and the mixed solution of Allicin and Alizarin.Respectively observed Trichomonas survival statusat 2h,4h,8h,12h,24h,calculate the parasite mortality rate.The 200 pigeons infected with trichomoniasis were randomly divided into four groups,mixed solution of Allicin,Alizarin set low,medium and high concentration group and the control group,feeding for 5 days.Result:Allicin was demonstrated the effects against Trichomonas vaginalis in vitro,The results oftrialed medicines on the Trichomonas vaginalis in vitro showed that minimal lethal concentrations(MLCs)of extracts of Allicin on the Trichomonas vaginalis was 0.5 mg/ml.When the drug concentration is above this concentration,with increasing concentration,the time of each experimental group 100%mortality of Trichomonas gallinae also will shorten,2mg/ml insecticidal rate reached 100%at 4h,with the control group comparison,the difference was significantly(P<0.05).Also Alizarin was demonstrated the effects against Trichomonas vaginalis in vitro,The results oftrialed medicines on the Trichomonas vaginalis in vitro showed that minimal lethal concentrations(MLCs)of extracts of Alizarin on the Trichomonas vaginalis was 0.75mg/ml When the drug concentration is above this concentration,with increasing concentration,the time of each experimental group 100%mortality of Trichomonas gallinae also will shorten,3mg/ml insecticidal rate reached 100%at 4h,with the control group comparison,the difference was significantly(P<0.05).In initial applications,cure rate of high and medium concentration group is up to 90%,While the cure rate of the low concentration group was 64%,the cure rate of control group was 0.