Identification And Characterization Of Histone Lysine Methylation Modifiers In Fragaria Vesca

Histone modifiers play an important role in the development of plant and animals.Histone lysine methylation is one of the important modifiers in histone modification,and plays vital roles in plant development.Several SET domain-containing genes were identified in Arabidopsis thaliana,and they can be further divided into seven classes with different domain construction and function.The sequenced genome of strawberry will facilitate the molecular research of strawberry.The researches of histone methylation and demethylation in Arabidopsis thaliana can be a guide for the study of histone modifiers in strawberry.Studies of histone methylation and demethylation in the development of strawberry could contribute to the breeding of strawberry.We performed both bioinformatic and molecular methods to identify the histone methylation and demethylation genes in strawberry.And we further analysed evolution and expression patterns of those genes.The main results are as follows:1.In total,we identified 45 SET domain-containing genes,22 JmjC domain containing genes and 4 LSD genes in strawberry.2.Phylogenetic analysis shown that SET domain-containing genes can be divided into seven classes,each of which has different domain construction and function.Except for the SET domain,SET proteins also contain other essential domains,e.g.PHD domain,AWS domain and post-SET domain.JmjC domain-containing genes can be divided into eight classes;genes within each class have similar domain and motif construction.The number of LSD genes are less than the JmjC genes,and LSD genes have a more conserved domain construction.Further analysis of LSD genes show that the genes divided into two groups in every species we selected.3.Exon and duplication of genes in strawberry were analyzed in our research.We provide evidence that whole genome duplication and dispersed duplications via retrotransposons may have played pivotal roles in the expansion of histone modifier genes in strawberry.4.Transcriptome data demonstrated that the expression patterns of SET genes are different at different stages or organs.Furthermore,the expression levels of some SET genes increase as the fruit develops and peaks at the turning stage.Meanwhile,we have observed that expression of those SET genes responds to cold and heat stresses.We performed comprehensive analysis on histone methylation and demethylation genes in strawberry,and expression of these genes during the development and stress treatment.Our study would shed light on the study of histone methylation in strawberry in the future.