Olfaction And Its Response Under Ocean Acidification Of Babylonia Areolata

For the characteristics of Babylonia areolata of fast growing,short breeding cycle,large individuals,high yield and tolence to hypoxia,it has become an emerging aquaculture speice.The Feeding behavior of Babylonia areolata has the phenomenon of fast resopnse,snatching and lycorexia.Limited information was available concering the olfaction and feeding ecology of this snail.Our study containe three parts:research on feeding behavior and olfaction related organs of Babylonia areolata,study on feeding attractant of Babylonia areolata and the effects of ocean acidification on feeding response,others are about growth and metabolism of Babylonia areolata.The main results are as follows:1.Research on feeding behavior and olfaction related organs of Babylonia areolataAccording to the foraging rhythm observation of Babaylonia areolata.We found that Babylonia areolata are nocturnal under natural condition meanwhile it will forage all day when feeding attractant exists.The nighttime movements are more active under starvation compared to the satiation.The feeding response to attractant significant reduced in few hours after feeding.The feeding response to attractant recovered about 6hrs after feeding.The results of behavioral and histological research of olfaction related organs show that rhinophore contributes nothing to feeding behavior and osphradium plays an important role in the initial phase of food finding and tentacle works in orientation.2.Research on feeding behavior and olfaction related organs of Babylonia areolataBabylonia areolata shows preference among four bait.The preference are Ruditapes philippinarum-Crassostrea angulata>Litopenaeus vannamei>>Decapterus maruadsi.The attraction acivities of twenty Lramino acid were tested using Attraction acivitity evaluation criterion.We found 8 kinds of amino acid(His,Pro,Cys,Val,Try,Asn,Ile)show no attraction acivities,6 amino acids(Met,Gln,Arg,Ser,Ala,Thr)shows low attraction acivities,6 amino acids(Leu,Phe,Tyr,Glu,Gly,Taurine)shows high attraction activities.The amimo acids combination shows significant higher attraction activity than single amino acids.Meanwhile we found the kinds and amount of releasing amimo acids of different baits has a certain correlation with their attraction acivities.It shows that amimo acid play a important role in feeding behavior inducing of Babylonia areolata.3.The effect of ocean acidification on feeding response,growth and basic metabolism of Babylonia areolataFeeding responses of Bablylonia areolata has no difference before 20 d.But feeding responses significant reduced between 20?30 d.The apocleisis tempurature of babylones under acidification condition is lower than control individuals for 0.5?.The electron microscope results show that the cilia on inner surface of rhinophore and on surface of tentacles are deformative developed.The oxygen consumption rate was not affected in day 45 and day 90 and ammonia excretion rate of 3000 ppm individuals was significant elevated compared to control individuals.The growth of babylones was affected under acidification environment.