Excavate Genes About Urea Regulating Arthrobotrys Oligosporus Trap Formation Based On Transcriptome Data

The previous research documented that feeding-bacteria can regulate nematode-trapping fungi killing nematode predator by producing urea.In this paper,transcriptome sequencing and Real time PCR were used to screen the candidate genes of urea-inducing trap formation in A.oligospora.The function of the gene Argl in the urea cycle was identified by knock-out and reverse-mutation.In addition,T-DNA random insert mutants in A.oligospora were screened by Agrobacterium tuwmefaciens-nediated genetic transformation(ATMT).The results are as follows:1.Based on the analysis of the transcript data of the different time of trapping formation induced by urea,there are 1970 different genes in T1 phase,which mainly clustered into the RNA degradation pathway,alanine,aspartic acid and glutamic acid metabolism and other amino acids and antibodies of the biosynthetic pathway.Furthermore,2665 genes in T? phase which mainly clustered into the amino acid biosynthetic pathway,RNA degradation and secondary metabolite pathway.2.Arginnase transforms arginine into urea and ornithine.To identify the function of gene Argl(AOL-s00083g426r)by homologous recombination and reverse,the results showed that there was no significant difference in the growth rate,mycelial morphology and stress resistance between the mutants and the wild type,and the sporulation was significantly lower than that of the wild type.The traps of ?Argl mutant decreased by 85%from arginine,meanwhile,the traps induced by nematodes and urea were slightly enhanced.For the reverse mutants,there was no difference in strain phenotype and trap formation conpared with the wild type.3.Three mutants,TP1,TP2 and TP3 were obtained by genetic transformation of ATMT.The sporulation of TP2 and TP3 was lower than that of wild type,whereas the sporulation of TP1 was increased,which was 2.6 times of the wild type.Moreover,the number of trappers by nematodes of TP1 and TP3 was lower than that of the wild type,respectively,decreased by 35%and 15%.