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Preference For Feeding Plants And Analysis Of Different Expression Of Olfactory Genes Of Batocera Lineolata

Posted on:2018-04-27Degree:MasterType:Thesis
Country:ChinaCandidate:Y YangFull Text:PDF
GTID:2393330542962762Subject:Forest Protection
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Batocera lineolata is a kind of woodworm insect that is very destructive to the economic,timber and ecological forest trees,whose larvae is stem-boring and adult is bark-gnawing.The injured trees are stunted or death seriously.Nowadays,the major measures are taken to prevent B.lineolata is spraying chemical agents,but chemical prevention and control can lead to environmental pollution.So it is a research hot spot for us to find an effective method for ecological control of B.lineolata.The forest investigation and real-time PCR were used in this study.The task is that exploring the host perference rules of B.lineolata,researching the different expression of olfactory genes of B.lineolata with different status,expounding the difference of olfactory expression rules and explaining the relationship between host prefenence characteristics and olfactory expression at gene level.The main results were follows:1.While B.lineolata was a sort of multi feeding insect,a lot of host plant species were suffered harmly by them.The survey showed that adults of B.lineolata prefered feeding fresh shoots of Rosaceae plants,for example,Pyrus spp,Rosa cymosa,Rosa multiflora and Rubus coreanus.On the other hand,the adults also liked feeding some other categories plants,like Cylobalanopsis glauca,Eucalyptus robusta and Juglans regia.It confirmed the significant positive correlation between the damage degree of Populus tomentosa and the proportion of Rosaceae plants(P<0.05).The female and male adults showed a strong preference on host plant C.glauca(P<0.05).But the plants of performance between P.tomentosa,Broussonetia papyrifera,Morus alba and Eriobotrya japonica were not obvious.At the same time,the survey showed that the forest edge samples were more feeded by the adults of B.lineolata.The degree of damage was significantly higher than those samples which were in the forest(P<0.05).2.The female and male adults of B.lineolata showed some little different preference on P.tomentosa,C.glauca,J.regia and E.robusta.The performance of B.lineolata as a whole followed in descending order,first was C.glauca,second was E.robusta,third was J.regia,and last was P.tomentosa.There were some differences in host plants perference between adults of female and male.The female adults showed a significant differences for 4 host plants(P<0.05).Male adults showed a significant differences for host plants P.tomentosa,C.glauca and E.robusta(J.regia)(P<0.05).The female adults did not likely more perference to the host plant J.regia than E,robusta.The B.lineolata adults showed no perference to feeding the host plant P.tomentosa.3.The results showed that the expression level of OBP and CSP of no feeding B.lineolata adults were the same with the reference gene expression,while the expression level of OBP and CSP of feeding adults were significantly increased.The expression level of olfactory genes of feeding B.lineolata followed in descending order,first was feeding host plant C.glauca,second was feeding host plant E.robusta,third was feeding host plant J.regia.There were significant differences in the expression of olfactory genes in male and female.Most of olfactory genes expression level of female adults with no feeding had no changes with the reference gene expression,but olfactory genes expression level of male adults were below the reference gene expression level.The expression level of 10 genes of female adults which feeding host plant E.robusta were higher than reference gene.OBP2,OBP3,Minus-C OBP1,CSP2 and CSP3 were increased significantly on male adults.The expression level of 10 genes of female and male adults which feeding host plant C.glauca were increased significantly(P<0.05).The expression level of 10 genes of female adults which feeding host plant J.regia had no different with the reference gene expression level,while 10 genes of male adults were higher significantly(P<0.05).
Keywords/Search Tags:Batocera lineolata, feeding host, perference, olfactory genes expression
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