| Bemisia tabaci is a devastating pest throughout the world,which causes serious damages to plant through directly feeding and indirectly transmitting kinds of geminivirus.Although Bemisia tabaci Q is not the vector of the plant virus TSWV(Tomato spotted wilt virus),there exists complex interaction between Bemisia tabaci Q and TS WV.TS WV influenced the settling and oviposition choice of B.tabaci Q on their host plants.Compared with the non-viruliferous Datura stramonium,B.tabaci Q showed a significant preference for TSWV-infected plant although such choice reduces its fitness.In the current study,we analyzed the acquisition and retention of TSWV on B.tabaci Q by q-PCR and RT-PCR,and then compared the fitness difference of viruliferous and non-viruliferous B.tabaci Q reared on cotton.Besides,the feeding behaviors of TSWV-infected and non-infected B.tabaci Q on cotton were also analyzed by electrical penetration graph(EPG).We aimed to assure the influence of TSWV on fitness,feeding behaviors and Tomato yellow leaf curl virus(TYLCV)transmitting of its non-vector B.tabaci Q.The main results were as follows:1.Acquisition and retention of TSWV on B.tabaci QIn the present study,the acquisition and retention of TSWV by B.tabaci Q were analyzed by q-PCR.We measured the relative concentrations of TSWV virion in B.tabaci Q after acquisition access periods(AAPs)of 4 h,8 h,12 h,24 h,48 h and 72 h.Results showed that the relative amount of TSWV was the lowest after 8h,while after 48h the relative amount of TSWV was the highest.We used RT-PCR to measure the transmission of TSWV by B.tabaci Q.After a APP of 48h on TSWV-infected plant,B.tabaci Q was transferred to non-viruliferous cotton plants and B.tabaci Q which was kept on cotton for 5 d,10 d,15 d,20 d and its next-generation adults were collected for detecting of TSWV.Results showed that TSWV can be detected on B.tabaci Q which was kept on cotton for 5 d,10d,15 d and 20 d,but can not be detected in the adults of the next generation.2.Effects of TSWV on B.tabaci Q fitnessWe compared the fitness of viruliferous and non-viruliferous B.tabaci Q reared on cotton.TSWV infection substantially decreased the adult longevity and fecundity of B.tabaci Q.In contrast,we found that TSWV did not significantly affect offspring survival,developmental duration,male and female body length between the viruliferous and non-viruliferous B.tabaci Q.3.Effects of TSWV on the feeding behaviors of B.tabaci QThe feeding behaviors of viruliferous and non-viruliferous B.tabaci Q were recorded and analyzed.Results showed that the duration of viruliferous B.tabaci Q salivation was 6.63 times longer than non-viruliferous B.tabaci Q and the number of salivation was 3.3 times greater than non-viruliferous B.tabaci Q.Besides,phloem ingestion phase of viruliferous B.tabaci Q lasted 2.95 times longer and the number of phloem ingestion was 2.94 times greater than non-viruliferous B.tabaci Q.However,there were no significant differences in two non-phloem parameters between viruliferous and non-viruliferous B.tabaci Q.We recorded the feeding behaviors of viruliferous and non-viruliferous B.tabaci Q on TYLCV-infected tomatoes.The duration of salivation of viruliferous B.tabaci Q was 3.37 times longer and the number of salivation probes was 2.5 times greater than non-viruliferous B.tabaci Q.However,phloem ingestion phase and the number of non-phloem probes did not differ significantly between the viruliferous and non-viruliferous B.tabaci Q.Moreover,there was no significant difference in the number of phloem ingestion and the duration of non-phloem between viruliferous and non-viruliferous B.tabaci Q.These results suggested that TSWV could promote the transmission of TYLCV. |
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