Mapping Of Resistance Genes To Stripe Rust In Wheat Landrace Dabaimai And Wheat-thinopyrum Intermedium Substitution Line P54

Wheat stripe rust,caused by Puccinia striiformis f.sp.tritici?Pst?,is one of the serious diseases on wheat,which had occurred many times in China and caused serious production losses.Using resistant varieties to control the wheat stripe rust is the most economic and effective method.Because common wheat and related species of wheat are the main sources of resistance genes to stripe rust,it is of great practical value to exploit and utilize the resistant resources for the wheat breeding,rational distribution of resistance genes and the prevention and control of wheat stripe rust.The materials Dabaimai and P54 were crossed with the susceptible variety Taichung 29 to construct the mapping populations respectively.Resistance identification was conducted to identify their resistant characteristics by the isolate CYR32 and 17126 of Pst,respectively.Finally,resistance genes of them were located by SSR and SLAF-Seq.The Chinese landrace Dabaimai was susceptible in the seedling and resistant at adult to CYR32,indicating there was the adult plant resistance?APR?in Dabaimai.Total 202 F₅ recombinant inbred lines of Taichung 29/Dabaimai were inoculated by CYR32.The genetic analysis showed that the Dabaimai carried at least 2 APR genes.Based on SSR markers and resistant identification,two resistance QTLs on chromosome 1AS and 4B,named QYr.caas-1AS and QYr.caas-4B,were detected using composite interval mapping method by WinQTLCart 2.5.For infection type,QYr.caas-1AS was located between the SSR markers Xgwm136 and Xcfd15 and the genetic distance from the marker Xgwm136 was 1.00cM.QYr.caas-1AS can explain the 45.97%phenotypic variance.QYr.caas-4B was located between the SSR markers Xwmc652 and Xgpw4388 and the genetic distance from the marker Xgpw4388 was 1.30cM.QYr.caas-4B can explain the 43.38%phenotypic variance.For disease severity,QYr.caas-1AS was located the same locus with SSR marker Xgwm136 and can explain the 26.83%phenotypic variance.QYr.caas-4B was located between the SSR markers Xwmc652 and Xgpw4388 and the genetic distance from the marker Xgpw4388 was 1.30 cM.QYr.caas-4B can explain the 21.30%phenotypic variance.The P54 was resistant to 17126 in the seedling,indicating there was the all-stage resistance.Total 206F₂ lines of Taichung 29/P54 were inoculated by isolate of Pst 17126.The genetic analysis showed that the P54 possessed two genes or three genes.Using SLAF-seq-BSA technology,P54,Taichung 29 and their F₂ generation were detected.Total 846,089 SLAF labels were obtained and 822,212 SLAF tags were anchored on the chromosomes.Through two methods of association analyses,264 genes were obtained,among which there were three non-synonymous mutational genes on 4A chromsome between the parents.The SSR technique was used to detect the resistance locus,which was located between the SSR markers Xgpw2302 and Xcfa2256 and the genetic distance from the marker Xgpw2302 was 4.00cM.