| Lignin is third most abundance biopolymers among all vascular plant and plays substantial role in stress response,mechanical support and water transport in plants.The expression,evolutionary and functional study of genes related with lignin biosynthesis provides an excellent model to study the coordinative evolution of a biochemical pathway in plants and helps to develop better strategies for bioenergy feed-stock improvement.In this regard,CRISPR/Cas9 technology which is a welcoming advancement in genome editing,owing to its efficiency,precision,ease of adoption and versatility.This study proposes that the agricultural fitness of the rice plant can be improved by modifying the H,S and G lignin monomers ratio.In addition,it is aimed to characterize four of the twenty seven lignin biosynthesis genes identified in rice by our laboratory,evaluating individual as well as collective events of reduced lignin,successfully developing CRISPR/Cas9 system to target lignin monomer biosynthesis genes,prediction of CAD1,C4H2,HCT1 and HCT2 proteins' 3D structures,comparative study of these genes with their homologous proteins present in Poaceae family,and checking productivity of transformed plants.Furthermore,this study will significantly play role in improving knowledge on composition of Secondary Cell Wall specifically in rice and developing improved approaches for biofuel production. |
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