Resistance Identification Of Kenaf Germplasm Resources Against Meloidogyne Enterolobii And Analysis Of Transcriptome

Meloidogyne enterolobii,was found by Yang Baojun in the Pacara earpod tree in 1983,compared to the common four species of root knot nematode,with stronger toxicity,stronger adaptability,can overcome the resistance of MI gene,its host range is also constantly updated.The fruit and vegetable of Hainan Island,the main hemp producing area of our country are being seriously harmed by this kind of nematode,however,the resistant material Meloidogyne enterolobii is deficient.On the basis of this study,220 kenaf germplasm Resources in China were tested in field disease resistance and pot resistance in greenhouse.According to the results of laboratory histopathological observation,the transcription sequence of root-knot nematode in several key infection periods was completed,and the sequence output of each sample was not less than 6Gb clean Data.The percentage of Q30 base is up to 85%.Complete the stitching and functional annotation of unigene,and carry out unigene CDs prediction,SSR analysis,SNP analysis,quantitative expression,differential analysis and functional enrichment analysis.The main results were as follows:(1)The results of field resistance identification showed that in 220 kenaf germplasm resources,there were 51 high resistant materials,32 high sensitivity materials,3 immune materials,and the results of pot resistance identification showed that in 220 kenaf resources,the high sensitivity material was 20,the high resistance material 106,and the immune material 11;(2)The results of indoor inoculation of representative high resistance and high sensitivity cultivars showed that the infection was the highest in the 18-hour inoculation,and the results were consistent in high resistance and high susceptible materials.(3)The results of several key period transcription group were analyzed,and a total of 81.95Gb clean data was obtained,each sample clean data reached 6.12gb,q30 base percentage of 89.69%and above.A total of 125,949 Unigene were obtained after assembly.There are 38,231 unigene in length above lkb.A functional annotation of the unigene,including a comparison to the Nr,Swiss-prot,Kegg,COG,Kog,go,and Pfam databases,results in a total of 82,499 unigene comments.Genetic structure analysis was carried out based on Unigene Library,in which 17,622 SSR markers were obtained.The expression of gene in each sample was analyzed.The differentially expressed genes were identified based on the expression of genes in different samples.Pattern clustering,functional annotation and enrichment analysis were performed on differentially expressed genes.(4)KEGG pathway enrichment analysis showed that susceptible and resistant kenaf germplasm genes differentially expressed significant mainly distributed in plant-pathogen interaction,plant hormone signal transduction,peroxide and RNA transport pathway,BAK1,WRKY33,IAA,AHP,ARR-A,PP2C,SnRK2,BAK1,CYCD3,TGASOD,PDCRRNase2,Exp5,eEF1A,eIF3,eIF2B,ACIN1,PABP are up-regulated,RBOH ARF EIN2,ATG1,ATG10,MPV17,HAO2,Um,eIF4E,gp210,UBC9 and THOC5 are down-regulated.