Proteomic Analysis Of Puccinellia Tenuiflora Calli In Response To NaCl

Monocotyledonous Puccinellia tenuiflora is a perennial halophytic species belonging to the genus Pu.ccinellia Gramineae.It is not only a good forage grass,but also a good model plant that to study the mechanism of salt-tolerance.Calli is a mass of undifferentiated cells which is made up of living parenchyma cells.It is the fist condition of gramineous plant regeneration and genetic transformation that sufficient mumber of callus are induced.Thus it is a good material of cell biology,molecular biology,plant embryology and developmental biology.Salinity affects plant production and ecological environment.Research on salt-tolerant plants mechanism of responsing salinity-alkalinity stress will provide valuable information for the use of technical means such as genetic transformation to improve varieties and enhance plant resistance.Here we investigated the metabolic mechanisms underlying salt tolerance of P.tenuiflora calli using a combined physiological and proteomic approach.The change of osmolytes,defense-related enzymes,the number and abundance of stress-responsive protines were analyzed in the calli in the course of salt stress treatment.In addition,we obtained 55 differentially abundant protein spots using two-dimensional electrophoresis(2DE)approach.They were mainly involved in carbon and energy metabolism,metabolism,stress and defence,protein synthesis,processing and transportation.These results included:(1)Osmopretectants of calli from P.tenuiflora were accumulated under salinity tolerance.(2)The antioxidant enzymes activitives were discrepant under salinity tolerance.(3)The energy generation decreased,Where the glycolytic pathway increased under salinity tolerance.(4)The protein synthesis increased under salinity tolerance.These provided more important information for further underlying the fine-tuned metabolic networks and regulative mechanisms in calli of P.tenuiflora.