Research On The Function Of Resistance Of ThERF4 Gene From Tamarix And Tamarix Androssowii Tissue Culture

ERF proteins playing an important role in abiotic stress in plants tolerance is one member of the AP2/EREBP large family,but the research on the function of ERF gene from Tamarix in the face of adversity is not clear.Then,we studied the mechanism of ERF gene from Tamarix when it responds to high salt and drought stress.At first,Our laboratory has optimized the transient transformation system and conclude that on the conditions of 1.0 OD of the concentration of Agrobacterium,0.05%Tween-20,120?mol/L of AS,the GUS gene expression and transient transformation efficiency is the highest.Next,by the use of the optimized transformation system,we transformed ThERF gene into tobacco for transient expression under salt and drought stress and conducted physiology staining,we screened out a gene,ThERF4,with strong anti adversity ability.Then we constructed ThERF4 gene of the overexpression and inhibition expression vectors,transform them into the Tamarix,and measured some physiological and biochemical indexes under salt and drought stress environment.DAB,NBT and Evans blue staining analysis found:ThERF4 gene has a good capacity of anti adversity and under the stress,its overexpression can reduce the ROS accumulation and cell membrane damage,but the inhibited expression of ThERF4 is just the opposite.Further physiological index shows that the expression of ThERF4 have positive correlation with the content of SOD,POD,proline,soluble sugar content,and negative correlation with the relative conductivity,H2O2 content,MDA content,water loss rate.This indicated that ThERF4 can regulate the SOD,POD activity to reduce the accumulation of ROS,regulate the synthesis of proline and soluble sugar to improve the osmotic potential to improve the ability of drought resistance and salt tolerance.We established tissue culture system of Tamarix and found two hormone combinations of differentiation medium which can promote the tamarisk stem segments from dedifferentiation,callus formation or adventitious root,and differentiation to ultimately the formation of complete plantlets.