| Coprophagy behavior is a special behavior of rabbits.The soft feces contains a large amount of dry matter,bacterial proteins and trace elements.The rabbits eat soft faeces can improve the feed efficiency and maintain the intestinal flora,which is very important for rabbits.In this study,a model of fasting faeces was constructed with New Zealand white rabbits,which were not fed feed before 30 days were used as research objects.Through feeding experiments and metagenomic sequencing,the effects of preventing faeces on the growth and development of New Zealand white rabbits were analyzed.In order to find out the flora associated with the growth and development of New Zealand white rabbits,it provides a reference for improving the production performance of rabbits.The test results are as follows:1.By constructing a preventing faeces model and raising it to 240 days,the slaughtered production indicators were measured.The results showed that there was no significant difference in feed intake of New Zealand white rabbits after fasting soft feces(P>0.05);In growth rate test,the group was significantly lower than the control group(P<0.05);at the end of the feeding trial,the final weight of the fasted feces group of New Zealand white rabbits was significantly lower than that of the control group(P<0.05);the total net weight and half net weight were extremely significant.Compared with the control group(P<0.01),the heart and liver of New Zealand white rabbits in the fasting feces group were smaller than the control group(P<0.01),and the kidney weight was significantly lower than the control group(P<0.01).P<0.05),there was no significant difference in spleen weight(P>0.05);the levels of triglyceride,high-density lipoprotein,and low-density lipoprotein in the fasting feces group had no significant difference with the control group(P>0.05).),but the total cholesterol content was significantly lower than the control group(P <0.05);in the index of fur quality,there was no significant difference in the hair length between the fasted feces group and the control group(P> 0.05),and the length of the fur,Width,weight,fasting feces group were smaller than the control group(P<0.05),fur Degree,soft feces fasting group than the control group was significantly(P <0.05);In the intestine index results,the fasted feces group was significantly lower than the control group(P<0.05),the length of the small intestine and ileum,fasting feces were significantly lower than the control group(P<0.05),and the cecum and colon did not differ significantly.Significant;By measuring small intestine slices,the results showed that the intestinal wall thickness and villus height in the fasting feces group were significantly lower than those in the control group(P<0.05),and genes related to intestinal development have been reported.The quantification in the small intestine tissues of two groups of rabbits revealed that the expression level of GHR gene was significantly lower in the fasted feces group than in the control group(P<0.01),and the expression level of INSR gene was significantly increased in the fasting feces group,lower than the control group(P<0.05).2.Through metagenomic sequencing of the gut microbiota of the cecum of New Zealand white rabbits in the fasting feces group and the control group at 240 days of age,a total of 474952702 reads were obtained.Species annotation of the obtained microbial genes was found and fasting was found.The abundance of Bacteroidetes in the excrement group increased,and the abundance of Actinobacteria decreased;at the genus level,the abundance of Bacteroides in the fasted feces group increased,and the abundance of Rumenococcus was abundance;KEGG annotation of gut microbial genes in fasted feces and control New Zealand white rabbits and found that microbial proliferation,glucose metabolism,and lipid metabolism pathways are rich in caecal microbial genes in both groups of rabbits The degree of collection was significantly different.Among them,riboflavin metabolism and bile secretion may be involved in the physiological activities of New Zealand white rabbits;through the correlation analysis of environmental factors and gut microbial genera levels,it was found that Oscillibacter and Akkermansia may be of New Zealand white rabbits.Weight and lipid metabolism have important regulatory effects.In summary,fasting feces significantly affected the performance of New Zealand white rabbits,as well as the development of organs,gut and fur,and lipid metabolism;and,metagenomic sequencing of cecal microbes showed that fasting feces may be possible.By altering the intestinal microbial diversity of the cecum,the lipid metabolism of New Zealand white rabbits is affected.The specific mechanism for this effect needs further investigation. |
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