Effect Of Mating Behavior On The Exocytosis Of Yeast-like Symbiotes In The Abdomen Fat Body Of The Brown Planthopper,Nilaparvata Lugens Stal

The brown planthopper,(Nilaparvata lugens,Stal)harbors a lot of yeast-like symbiotes(YLS)in the abdomen fat body.YLS play an important role in the growth,development and reproduction of their host,the brown planthopper(BPH).YLS can be transmitted from mother to offspring through transovarial transmission.The process can be reformulated with more detail as follows:YLS in mycetocytes moved out of the abdominal fat body and released into hemolymph by exocytosis.The free YLS in hemolymph reached the ovarian tube and entered the follicle cells around the primary oocyte by endocytosis at epithelial plug of the ovariole.However,there are few reports about the YLS transmission mechanism.In order to study effect of mating behavior on the exocytosis of yeast-like symbiotes in the abdomen fat body of the brown planthopper,Nilaparvata lugens Stal,the number of YLS in the chest,head and legs within 72 hours after emergence of the female brown planthopper under unmated and mated conditions by hemacytometer and real-time quantification PCR was determined.The community diversity of endophytic fungi in unfertilized eggs and fertilized eggs of brown planthopper was performed by high-throughput sequenceing technology,The differences in the number and species of YLS were analyzed and compared.The results are as follows:1.The number of YLS in the chest,head and legs within 72 hours after emergence under unmated and mated conditions was determined by hemacytometer.The results showed that with the growth and development of the brown planthopper,the number of brown planthoppers that YLS were detected increased significantly.The numbert of YLS under mating conditions was higher than that under unmated conditions.It showed that the mating behavior could promote the exocytosis of YLS in the fat body of brown planthopper.2.qPCR results showed that Within 72 hours after emergence,Pichia guilliermondii and two uncultured fungi which called 230 and 305 were not detected.Four YLS(Pichia guilliermondi,Hypomyces chrysospermus,230 and 305)were detected in the haemolymph of the brown planthopper under mating condition.It indicated that mating behavior play a key role in the exocytosis of Pichia guilliermondii,230 and 305 in the fat body entering into the hemolymph.Under unmated and mated conditions,the number of YLS in chest was the highest,then in head,the least is in legs;the number of Hypomyces chrysospermus was significantly higher than the other three YLS,and the number of other three YLS was not significantly different.The number of Hypomyces chrysospermus detected in the hemolymph under mating conditions was significantly higher than that under unmated conditions.It indicated that the mating behavior will promote the abdominal fat body of the brown planthopper to exocytosis a variety of YLS into the hemolymph.3.The study on the diversity of endophytic fungi harbored in unfertilized eggs and fertilized egg was performed by high-throughput sequencing technology.The results showed that:Mating behavior affected the endophytic fungi communities significantly.The species abundance of the endophytic fungi from Fertilized eggs were higher than unfertilized eggs.Ascomycota,Sordariomycetes,and Hypocreales were the main groups on each taxonomic category.There was no significantly difference in species composition on each taxonomic category between the unfertilized egg and the fertilized egg endophytic fungal community,but there was a significantly difference in the abundance of most species.