Mechanism Of Zearalenone-Induced Mitosis Catastrophe And Apoptosis Of Endometrial Carcinoma Ishikawa Cell

Zearalenone(ZEA),also known as F-2 toxin,is a non-steroidal mycotoxin(macrocyclic lactone)produced by several fungi of the genus Fusarium and is present in grains such as corn。The detection of the common pollutant in daily life is very tedious.As an exogenous estrogen-like mycotoxin,ZEA can exert estrogen-like effects in vivo,and in mammals,after some complicated biological processes,there will be two kinds of chemical organic derivatives,among which α-ZEL The toxicity is 10 times stronger than that of β-ZEL,and its metabolites not only have strong toxicity but also have a synergistic effect with the ZEA body and can more effectively exert a series of reproductive toxicity and estrogen-like effects of ZEA.At present,a large number of investigations and experiments have been done on the toxicological environment and estrogenic effects of ZEA.However,the mechanism of ZEA-mediated targeting of reproductive cells is too complex,and this effect can inspire us to study ZEA.The toxicological study of this toxin on the corresponding reproductive target organs is a well-recognized material for toxicology of reproductive and developmental research.The reproductive system,one of the most important systems of organisms,has been increasingly damaged by reproductive tumors in recent decades.Therefore,this study will reaserch the epithelial cells of Ishikawa cells,one of the female malignant tumors.Light microscopy,CCK-8 cell viability assay,DAPI nuclear staining,cell reactive oxygen species detection,cell mitochondrial membrane potential change detection,flow cytometry,human digital gene expression profiling(DGE)data analysis,real A series of experimental techniques,such as fluorescence-quantitative techniques and Western blotting,were used to study the morphological changes,viability values,proliferation and apoptosis,cell cycle changes,cellular and molecular levels,changes in protein levels,and regulation of cells.To explore the synergistic action of zearalenone-induced mitotic catastrophe and apoptosis of zearalenone cells to inhibit their proliferation.The results :(1)Low concentration(1~10μM)of ZEA did not inhibit the proliferation of human endometrial carcinoma Ishikawa cells,but at 10μM increased the proliferation of human endometrial carcinoma Ishikawa cells at 48 h.(2)High-concentration(30~100μM)ZEA significantly inhibited the proliferation of human endometrial carcinoma Ishikawa cells.The changes in cell morphology were evident,there are significant mitotic catastrophes such as increased shape,increased adherenceand polyploidy.High concentration of ZEA may induce Ishikawa cells to undergo mitotic catastrophe and apoptosis and inhibit cell proliferation by regulating the expression of related genes such as p21、Cyclin B1 in the periodic signal pathway and regulating the expression of downstream and other related proteins.Conclusion: ZEA can slightly promote cell proliferation in human endometrial cancer Ishikawa cells at low concentrations,and significantly inhibit proliferation at high concentrations,and cell cycle arrest at G2/M phase.