| To explore the effects of supplemental blue and UV-A on the growth,qualityof pakchoi(Brassica campestris L.ssp.chinensis Makino)and Chinese kale(Brassica albograbra Bailey)babyleaf,4 wavelength(380 nm,400 nm,430 nm,462 nm,40w/m~2and12h/d)LED were supplied in greenhouse.And to study the effects of blue LED lighting durations on the growth,qualityof pakchoi babyleaf,4 treatment(during 12 hours in daylight,no light:CK,15 minutes per 75 min(T1),1 hour per 2 hour(T2),and 12 hours(T3))were applied.1.The effects of different wavelength LED supplement on the growth,qualityof pakcho i and C hinese kale babyleaf.(1)Under supplemental Illumination of different wavelength of blue and UV-A LED,the growth and biomassof pakchoi and Chinese kalebabyleaf significantly increased.And the effect of blue light(430 nm,465 nm)was more significant than that of UV-A(380 nm,400nm),while there was no significant difference between 2 blue light treatments.Fresh weight of pakchoi and Chinese kalebabyleaf significantly increased 73.73%and 74.07%in430 nm treatment.The content of soluble protein inpakchoi and C hinese kalebabyleaf significantly increased under LED supplement,while there was no significant difference among treatments.The content of soluble protein inpakchoi and Chinese kalebabyleaf significantly increased by 17.09%and 18.15%respectively 430 nm treatment.And the content of soluble sugar inpakchoi and C hinese kalebabyleaf significantly increased by20.44%and 34.60%respectively in 430 nm treatment.(2)The pigment content in pakchoi and Chinese kalebabyleaf in blue LED treatment increased more than UV-A treatment.Under the illumination supplement of 430 nm,the content of anthocyanins,carotenoid and chlorophyll significantly increased by 22.61%,79.57%and 24.00%inpakchoibabyleaf,and significantly increased by 20.00%,145.51%and 29.52%in C hinese kale babyleaf.(3)The content of functional components in pakchoi and C hinese kalebabyleaf in blue LED treatment increased more than UV-A treatment.Under the illumination supplement of430 nm,the content of Vitamin C,vitamin E,kaempferol,quercetin,and polyphenols inpakchoi significantly increased by36.87%,7.71%,119.34%,13.07%and 37.11%,and the content of Vitamin C,kaempferol,and polyphenols inC hinese kale significantly increased15.20%,150.44%,and 20.37%.O nly in 400 nmtreatment,total glucosinolatescontent inpakchoibabyleaf significantly increased by 17.79%,andthe content of 4-methyl sulfonyl butyl glucosinolates,3-butenyl glucosinolates and 4-methoxy indole-3-methyl glucosinolatesincreased 15.13%,117.21%and 7.69%respectively.The content of glucosinolates inC hinese kalebabyleaf of 430 nm treatment was the highest,increased by283.74%.And the content of 2-propylene glucosinolates,3-butylene glucosinolates,indole-3-methyl glucosinolates,4-methoxy indole-3-methyl glucosinolates,and 1-methoxy indole-3-methyl glucosinolates significantly increased by 155.26%,737.78%,680%,85.71%and 307.41%respectively.(4)Under supplemental blue and UV-A,the content of phosphorus,calcium,magnesium,zinc and ferrum inpakchoibabyleaf and the content of calcium,magnesium,zinc,potassium and ferruminC hinese kalebabyleaf reduced,while the potassiumcontent inpakchoibabyleaf and the content of sulfur inpakchoi and C hinese kalebabyleaf were not significantly changed.The increase of mineral elements accumulationin pakchoi and C hinese kalebabyleaf were in sequence of430 nm,465 nm,380 nm and 400 nm.In 430 nm treatment,the accumulation of total phosphorus,total potassium,total calcium,total magnesium,total sulfur and total ferrum inpakchoibabyleaf significantly increased by 66.67%,75.93%,30.00%,40.39%,90.00%and 34.23%,and the accumulation of total phosphorus,total potassium,total calcium,total magnesium a nd total sulfur inC hinese kalebabyleaf significantly increased by 50.00%,36.00%,7.69%,11.46%and 36.36%.(5)Under supplemental blue and UV-ALED,the antioxidant capacity of pakchoi and Chinese kalebabyleaf enhanced,and the antioxidant capacity of pakchoi and C hinese kalebabyleafin blue light treatments was significantly higher than that of UV-A.In 430 nm treatment,the enhance of antioxidant capacity was most significant,After the illumination supplement of 430 nm,free radical scavenging rate of DPPH and FRAP value of pakchoi were increased by 43.44%and 26.15%,while free radical scavenging rate of DPPH and FRAP value of Chinese kale were increased by 16.67%and 19.19%respectively.2.The effects of different blue light supplementalschedule on the growth,quality of pakchoi babyleaf.(1)The growth and quality of pakchoi babyleaf significantly improved under blue light supplement,The highest biomass and content of soluble sugar and soluble protein inpakchoibabyleafwere found in T3 treatment.The fresh weight of pakchoi babyleaf increased 12.87%,38.46%and 91.24%in treatment of T1,T2 and T3.And the content of soluble sugar and soluble protein inpakchoi babyleaf increased 9.32%,9.45%and 23.13%,38.10%in treatment of T2 and T3,respectively.(2)Leaf color of pakchoi babyleaf was significantly affected byblue light supplement,the pigment content increased with the extension of illumination time.The anthocyanin content of pakchoi babyleaf significantly increased 24.64%in T3,while no significant difference was found in other two treatments.The content of carotenoid and chlorophyll inpakchoi babyleaf significantly increased 142.26%,322.70%and 15.07%,16.89%in T2and T3 treatment respectively.With the extension of illumination supplement,the brightness and chroma of leaves inpakchoi babyleaf decreased and red and blue deepened.(3)With the increase of illumination time,the content of phosphorus,calcium,and zinc inpakchoi babyleaf decreased.while no significant effect was found in the content of sulfur and ferrum.(4)With the extension of illumination supplement time,the content of vitamin C,polyphenols,kaempferol and quercetin inpakchoi babyleaf increased.The content of vitamin Csignificantly increased 10.33%in T3 treatment.The kaempferol content increased13.61%,24.78%,and 40.90%in treatment of T1,T2 and T3 respectively,and the content of polyphenols and quercetin increased 14.29%,10.88%and 14.61%,14.60%in treatment of T2 and T3respectively.(5)With the extension of illumination supplement time,free radical scavenging rate of DPPH and FRAP value inpakchoi babyleaf increased.Free radical scavenging rate of DPPH and FRAP value increased 17.79%and 14.84%in T3 treatment respectively.In summary,there was more improved effect for growth,quality and functional ingredients of babyleaf in blue LED than UV-A LED,andthose in 430 nmblue light were more significant.To light energy consumption considerations,6 hours per day light supplement(T2)was more economical for improvement of biomass,quality,and the content of functional components. |
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