Genome-wide Association Study Of Rice Blast About The Worldwide Collection Of Rice Germplasm

Rice blast is one of the ‘big there'disease in rice(Oryza sativa L).Recently,the new locus were detected by mapping and molecular cloning.In this study,we used the genome-wide associated study to identify the new rice blast area in the nature population.It is great theoretical and practical significance to establish the rational distribution system of resistance resources in different regions.In this study,586 international rice germplasm accessions from IRRI were identified by 5 isolates,and 700 K high density SNP(single nucleotide polymorphism)was used to explore the new loci about the rice blast,and screen high resistance cultivars.Three isolates(YN485,YN661,YN716)came from Yunnan province,which is the rice blast diversity center.Other two stains RB22 came from Zhejiang province and RO1-1 came from Korea.All the isolates were strong pathogenic.The results are as follows.1.After the spray inoculation and analyzed,we got 55 high resistant cultivars.According to the international standards that its resistance degree is less than or equal to 2.2.In TEJ sub-population,an average of 77.08%rice varieties were susceptible.In ADMX sub-population,an average of 52.82%rice varieties were susceptible.In AUS sub-population,an average of 49.5%rice varieties were susceptible.In ARO sub-population,an average of 47.8%rice varieties were susceptible.In IND sub-population,an average of 34.33% rice varieties were susceptible.In TRJ sub-population,an average of 27.38% rice varieties were susceptible.The TEJ sub-population were more susceptible than the TRJ sub-population.3.A total of 96non-redundant resistant loci were obtained by genome-wide association analysis,of which 11 sites belonged to repeat regions.174 candidate R genes were obtainedand been divided into 8 categories,of which transcription factor genes accounted for 27% and the LRR-related genes accounted for 13.2%.incompatible with4.The quantitative Real-time PCR was used to verify the expression level of the resistance gene.According to the RNA-seq data we identified that 9 candidate genes were up-regulated expression in incompatible interaction,and 97 candidate genes were the up-regulated expression in compatible interaction.6 of them were consistent with the data of transcriptome expression level used the QRT-.PCR.This study used the lager population and several isolates to identify the new locus.These results lay a foundation for study on excavating the new rice resistance gene,and it give a prevention and control of the rice blast.This study also provide a new way to identify the resistant cultivars.