Survival Characteristics In Soil Of Fusarium Oxysporum F. Sp. Cubense And Its Relationship With Microbe

Banana,family Musaceae,genus Musa,is one of the world's most important fruits.There are more than 130 countries in the world that produced bananas,with the largest production in Central America,followed by Asia.Banana Fusarium Wilt is a very serious soil-borne disease caused by Fusarium oxysporum f.sp.cubense(FOC).It would results in low the survival rate of the banana,and is serious ly harmful to the development of the banana industry.Therefore,this study investigates the survival characteristics of pathogens in the soil and the relationship with microorganisms,so as to clarify the pathogen in the soil epidemic law,lay the foundation for effective prevention and control of diseases.A total of five degrees of banana diseased soils were be selected.The same concentration of banana FOC race 4 was used to make the slides,and the cells were inoculated at 0 d,10 d,20 d,30 d.The effects of different methods on the growth and development of banana fusarium wilt race4 in different soils were studied.The method was analyzed by fluorescence quantitative PCR,plate counting method and Miseq sequencing.Analyzing the variation pattern of FOC4.In different types of soil,and the diversity and community structure of various fungi and bacteria in soil.The results showed as follows:(1)After inserted the FOC4 spores manually into five different soils A,B,C,D and E,the conidia could germinate and grow mycelium on the day of inoculation.After 10 days of inoculation,the conidia were found,and a large number of microconidia,after 30 days of inoculation can see chlamydospore.The number of spores in the field of view first increases after spore germination,and then gradually decreases.(2)The amount of original soil was A <B <C <D <E,which was consistent with the incidence of field banana fusarium wilt.After inoculation of FOC4 spores,the amount of original soil and inoculated at different times was quantified by plate counting method.The results were consistent with the results of qPCR quantification and were at the same order of magnitude.The decrease efficiency of FOC4 content in five soils was analyzed.The descending efficiency of the five soils was the lowest at 10 days after seeding,and then began to drop significantly.The dose gradually reached the gentle period after 20 days of inoculation,and then decreased gradually.(3)The Burkholderiales and Legionellales were negatively correlated with the incidence of banana fusarium wilt in the bacterial community,and the Rhizobiales,Acidobacteriales and Pirellulales were positively correlated with the incidence of Banana Fusarium Wilt.In the fungal community,the Hypocreales was positively correlated with the incidence of banana fusarium wilt.(4)Inoculation of soil at different time intervals,10 d after inoculation is an important time node,after which the bacteria Burkholderiales,Legionellales and Rhizobiales significantly reduced.There was no significant increased trend which Pirellulales inoculated after 10 days.The results also showed that there was significant recuded trend which Hypocreales inoculated in the soil C,D and E after inoculating for 10 days,and there was no significant difference in the efficiency of FOC4 in soil A and B.