| Wheat rust is one of the most common and widespread diseases caused by wh eat rust.It occurs frequently and can cause serious losses.Although chemical rot c an effectively control leaf rust,it can be cultivated and promoted.Disease varieties are the more economic,effective and environmentally friendly ways to prevent and control wheat leaf rust.The expression of resistance depends on the host-host inter action,environmental conditions,plant growth stages,and the interactions between t he resistance genes in the wheat genome.Most of these genes are gene-specifically conferred on the race-specificity of wheat rust-resistant bacteria.Sex resistance.Ho wever,wheat varieties that rely on race-specific resistance often lose efficacy within a few years.Large-area planting of susceptibility disease wheat varieties facilitates the propagation of large numbers of rust populations.Therefore,the search for ne w disease-resistant genes and new source-resistance materials,the acquisition of mol ecular markers closely linked to them,and the use of molecular marker-assisted sel ection techniques for the accumulation of disease-fighting genes are important for th e breeding of wheat cultivars with lasting disease resistance.significance.This pape r tests and analyzes wheat rust through the following two parts:In the first part of the study,80 wheat varieties from North America,Russia,CIMMYT,and Henan Province were identified for field resistance using genetic der ivation and molecular marker techniques to determine the field resistance of each ru st resistance gene and obtain a phenotype.Data were simultaneously tested with mo lecular markers and genotype data were obtained.The combination of the two provi des a genetic basis for the breeding of resistant rust-resistant varieties.In the experi ment,20 leaf rust races with different virulence were used to inoculate these 80 w heat cultivars at the seedling stage and were identified for seedling stage resistance,compared with 36 vector varieties containing known leaf rust resistance genes.In combination with marker detection,seven genes including Lr1,Lr26,Lr17,Lr14 a,Lr13,Lr39 and Lr46 were deduced from the test materials.21 of them contained L r1,41 of them contained Lr26,5 of them contained Lr46,26 of them contained L r13,20 of them contained Lr14 a,27 of them contained Lr17,and 22 of them contained Lr39.Lr9,Lr24,Lr19,Lr28,Lr33,Lr47,Lr51,and Lr53 are all absent from all materials.The second part of this study was to analyze the QTL for resistance to stripe rust in adult plants of variety W014204.It has good agronomic traits,and adult pla nts have good rust resistance to leaf rust and stripe rust.Therefore,in order to locat e the adult rust resistance genes in this trial,216 recombinant inbred lines that wer e crossed and selfed by W014204/Zhengzhou 5389 were planted in Baoding,Hebei,in 2015-2016 and 2016-2017 respectively.In Mianyang,Sichuan,four highly virul ent striped rust mixed races such as MHJS,THJL,PHGP,and THTT were used to collect phenotypic data for the disease severity survey in the spring.In the 216 re combinant inbred lines,five lines were selected to establish a small population with resistance,and SSR and SNP markers were used to screen between the parents an d small groups to select markers with an exchange rate of 30% or less.To further test the large RIL population,read the band type and obtain the genotype data.Q TL analysis was then performed on the obtained phenotype data and genotype data using mapping software QTL ICI mapping 3.1 and MapManager QTXb20.The res ults showed that among the 1100 SSR primers currently available in the laboratory,the exchange rate of 10 pairs of primers was selected to be less than or equal to30%.The results after software analysis showed that a QTL site for resistance to stripe rust on adult plants was mapped on chromosome 7DS.The marker interval g wm130-cssfr1 was confirmed to be Yr18,explaining a phenotypic variation of 9.6%-14.3%. |
PDF Full Text Request