The Research Of Poplar Resistant To Botryosphaeria Canker

Canker of poplar harm more and more serious,especially for plantation caused widespread influence,which lead to the healthy development of the plantation industry constrained and the decrease of the quality of the wood products and forestry economic benefits suffer heavy loss.At present,in view of the poplar canker there is not drug can effect a radical cure,and only rely on the traditional cross breeding way to select new varieties,and through the indoor inoculation of new clones ulcer bacteria and field production environment survey to screen new clones of ulcer disease resistance is strong.Paper to filter out stronger resistance to canker of clone as the purpose of the test,Experiment with the‘02-9-22'?‘02-8-21'?‘02-12-29'?‘02-21-13'?‘02-3-32'?qinbaiyang3?‘03-6-11'?‘03-4-9'?‘03-4-22'?‘03-5-17'?‘04-16-10'?‘04-19-14'?‘04-17-12'?‘04-14-15'?Populus alba×p.tomentosa?I-101?84K?P.populus tomentosa No.30?P.populus tomentosa No.85?08H-4-5'?‘08H-1-6'?‘07-69×qing1'????1??Populus 69?‘08H-11-11'?‘06H-6-9'?‘03-south4-1'?Shanlin No.3?‘07H-6-23'?‘06-57×chuan 1'?Shanlin No.4?‘07-xidazhai×bo 1'?‘06-69×bo 1'33 clones or varieties as the test material.The experiment on the branches of the indoor inoculation test and outdoor environment the growth of the field survey,and integrated the two results of indoor and outdoor,to analysis level,clone sense of poplar canker disease on the screening of the strongest clonal plant disease resistance.In order to the future of new varieties of poplar canker resistant breeding work provides the theoretical basis;Test results are as follows:1.33 of P.tomentosa and P.deltoides and P.deltoide×P.cathayanna poplar clones or production of varieties of experimental forest twice the poplar canker of field investigation,observation and record of poplar branches on the number of disease spot,measuring each of poplar diameter at breast height,and then calculate the disease index,analysis of various of poplar resistance between significant differences and growth.Experimental results show that P.tomentosa new clones or varieties' 03-4-22 '?P.populus tomentosa No.85?'04-16-10' production field of canker in the form of high level,but its less average diameter at breast height and it were 16.43 ± 1.98 cm,13.7 ± 1.85 cm,1.85 ±2.96 cm;Clones' 02-21-13 'average diameter at breast height,the largest is 19.71 ±1.76 cm,but its relatively low resistance index of cankerous disease,characterized by moderate level of infection;The clones' 03-6-11 'relative resistance of canker is higher and its average diameter at breast height size was 18.32±2.34 cm,breeding of poplar fast-growing and are sent to canker resistant clones were high;P.deltoides and P.deltoide×P.cathayanna poplar field survey by cathay poplar clones' 08H-4-5 'resistance index and investigating the highest average diameter at breast height,the largest second resistance better for poplar 69,' 08 H-1-6,clones' 06-69 × qing 1 'relative resistance index of canker min,and it is the high canker resistance clone.2.P.tomentosa 19 clones or variety of in vitro shoot inoculation ulcer diseases(Botryosphaeria dothidea),periodic observation of in vitro shoot inoculation point the onset of infection situation and record in vitro branches points,calculate the resistance index of branches,the experimental results showed that poplar clones or varieties ‘03-6-11' ?‘04-16-10'?‘02-9-22'and Populus alba×p.tomentosa were the highest canker resistance clone;‘03-4-9'?I-101?‘04-19-14'?‘03-4-22'?qinbaiyang 3?‘03-5-17'?‘02-8-21'?‘04-17-12'?84K were the resistance clone.P.populus tomentosa No.30?‘02-12-29'and‘02-21-13'were the susceptible clone;‘02-3-32'and‘04-14-15'were the highest susceptible clone.Clones' 03-6-11 ','04-16-10','02-9-22' disease index is relatively low,can be used as the preferred material for poplar disease resistance breeding new varieties.3.To 14 varieties of P.deltoides and P.deltoide×P.cathayanna poplar clones or branches in vitro inoculation ulcer diseases,the results showed that the clones' 08H-4-5 'disease index is relatively low,for high clone;Variety poplar 69,clones' 08H-2-16 ','08H-1-6',‘08-69×qing 2'?‘07-69×qing 1'disease index is relatively high,as the resistant clone;Other clones for high canker resistance clones.From the test results,clones' 08 H-4-5 'is superior to the control of the disease resistance,can become disease resistance breeding of superior clones.