Study On Distribution Of Yield Traits Genes And Rice Blast Resistance Genes In Japonica Rice Resources In Northeast Of China

In recent years,with the continuous development of molecular marker technology,molecular marker-assisted selection(MAS)technology combined with traditional breeding has played an important role in rice breeding for new varieties.For a long time,the goal of breeding high-yield varieties and raising rice yield breeders has been in the northeast region of China.The frequent occurrence of rice blast disease has restricted the high yield of rice.Therefore,it is important to breed resistant varieties.The purpose of the study was to analyze functional genes DEP1? Gn1a? qGW8? GS3?GW2? IPA1 and qSW5 related to the yield traits of some green traits,and the blast resistance genes Pid2?Pid3?Pi9?Pi2?Pizt?Pita?Pi5 and Pib in 339 Japonica rice(102 rice varieties in Heilongjiang,81 rice varieties in Jilin,and 156 rice varieties in Liaoning),and the analysis of these important genes Japonica rice breeding in northeast China through the detection of functional gene loci that control green traits applied value and contribution.1.The distribution of seven yield traits related genes in 339 japonica rice was tested.The allele variation was not detected in the ideal plant gene IPA1 and the grain wide and grain weight GW2?DEP1?qGW8?GS3 and Gn1 a were detected 2 alleles,and three allele variations were detected in qSW5.DEP1 was rarely used in the Jilin and Heilongjiang cultivars,accounting for only 4.93% and 7.84%.Time goes by,DEP1 was detected in Liaoning cultivars(80.70%)and the number of cultivars carrying the DEP1 gene increased;GS3 has a small amount of distribution in resources,and it is mainly distributed in Heilongjiang breeds in 2001-2011 and after 2012.The Gn1 a gene was introduced and utilized only in a small number of Liaoning varieties,which focus on varieties popularized after 2012;qGW8 is mainly distributed in Liaoning Province and it has relatively little use in Jilin Province,while a little Heilongjiang rice material carries qGW8.qSW5 can be detected in all three regional breeds and can be detected in each promotion period in recent decades.qSW5 has been fixed in the northeastern area.2.8 rice blast resistance genes were tested and identified for resistance to 339 Japonicarice resources in northeast of China.Pi9 and Pi2 genes were not detected in 339 Japonica rice resources.Pizt? Pid2?Pid3?Pita?Pib and Pi5 appeared in different regions with different detection rates;When rice varieties carry only a single rice blast gene,they are all poorly resistant to rice blast,but when combined with other genes,rice varieties are resistant to rice blast.It has been improved in different degrees.In the present study,the combinations of valuable disease resistance genes for resistant to rice blast were different in the three regions.Pi2?Pizt?Pita and Pi5 contributed much to the resistance to rice blast in Heilongjiang,but when Pita and Pi5 were polymerizated,the resistance improvement of Heilongjiang rice was the most significant;Pid2+Pid3+Pita was the most greatest combination that contributed to the improvement of rice blast resistance in Jilin Province;Pita+Pi5 was the greatest contribution to rice blast resistance improvement in Liaoning Province.In this study,7 functional traits related to yield traits in 3 regions of Northeast China were tested,but no traits carrying these 7 functional genes were detected at the same time.The rice blast resistance of different combinations of genes was identified under natural conditions and artificial inoculation conditions,which is of great value for preliminary screening of excellent germplasm resources and breeding of disease-resistant varieties.