Influence Of Genetic Background And Ecological Environment On The Character Of Vascular Bundles Of Rils Derived From The Cross Between Indica And Japonica

The vascular bundle is an important part of the rice“source,reservoir,and stream”system,and it bears the functions of transportation of mineral elements and organic matter,and has a greater impact on the panicle traits,and is closely related to lodging resistance.Rice vascular bundle traits There are significant subspecies differences.In this experiment,155strains of F₁₀ generation of recombined inbred lines?RILs?constructed from indica rice cultivar Shennong 265 and indica rice cultivar Ruhui 99 were used as tested materials and planted in different ecological environments under different ecological environments(South China The japonica rice region of Shenzhen in Guangdong Province,Deyang in Southwest China's Japonica Region,Huai'an in Jiangsu Province in the middle and lower reaches of the Yangtze River,and Shenyang in Liaoning Province in the northeastern region of China.We analyzed the frequency and ecological environment of the vascular traits in rice.Impact and relationship with panicle traits.The results of the study are as follows:1.The characteristics of large vascular bundles in different regions conform to the trend of Huai'an>Deyang>Shenyang>Shenzhen,and the number of large vascular bundles of Panicle in Huai'an and Deyang regions is significantly higher than those in Shenzhen and Shenyang.The difference of small vascular bundles is similar to that of large vascular bundles.Both Huai'an and Deyang are higher than Shenyang and Shenzhen.The correlation between frequency of indica alleles and large vascular bundles has reached a significant level,so the large vascular bundles in different regions are not only affected by the ecological environment,but also affected by the frequency of indica alleles itself;while the frequency of indica alleles is not significantly related to the small vascular bundles.Therefore,the difference of small vascular bundles in different regions may be due to the impact from the ecological environment.There was a significant difference in the LVB ratio between the last two sections and the phenotype of the burrow-neck vascular bundle,but it was not related to the frequency of the pupa type.It was speculated that the LVB ratio was more easily affected by the environment.There was a difference between the size of vascular bundles in the panicle neck?LVB/SVB?and the efficiency of primary branches?V/R?,but these two traits were significantly positively correlated with the frequency of pods.The effects of the traits are greater than the ecological environment.2.Construct a linkage map of 3569 Ape-specific SNPs,and compare the ratio of large vascular bundles,small vascular bundles,and vascular bundles?LVB/SVB?in the panicle and neck of the four regions,and the two major sections of the panicle and neck section.The phenotypic data of LVB ratio and primary branching efficiency?V/R?were used for QTL analysis.Two QTLs affecting the large vascular bundles of the panicle neck were located on chromosomes 6 and 9 respectively,and were located in multiple regions simultaneously;one LVB ratio was detected on chromosome 6 in all four regions;V/The QTLs of R have more loci,but the consistency is lower.This may be due to V/R being affected by both branch number and vascular bundles.It is worth noting that the location of the QTL on the chromosome 9 of the panicle-neck vascular bundle is similar to that of the known erect panicle-type gene DEP1.Whether DEP1 is polymorphic to vascular bundle traits requires further study.3.The results of correlation analysis showed that the numbers of large and small vascular bundles and panicle-necked knots,and the number of primary branch stems,number of primary branch stems,number of secondary branch stems,and number of secondary branch stems in the second and third sections of different regions were all present.Significantly positive correlation was found;there was a highly significant negative correlation between the pod diameter of the vascular bundles in the panicle neck and the secondary branches,and the ratio of the LVB ratio to the number of primary branches and the number of primary branch pods in the second and the last section.There was a significant negative correlation between the number of secondary branches and the number of secondary branches.4.Analysis of results of vascular bundles,branches,and related traits in different parts of the cobs showed that the primary branch stem efficiencies?V/R?were consistent in different regions.All of them were of hemiparalysis type>intermediate type>hemic hemisphere type,lower V/R>middle V/R>upper V/R;the lower V/R is generally larger than the whole ear,while the upper V/R is generally smaller than the whole spike,and the middle V/R is basically the same as the whole spike;different types of pods and spikes The difference in the traits of primary branch traits at different parts of the plant was less than that of the secondary branch stalks,especially the secondary branch stalk number and the secondary branch stalk seed setting rate.Further analysis found that the number of large vascular bundles in each primary branch stem gradually decreased from the bottom up,higher V/R values resulted in a lower seed setting rate,especially for secondary branch stems.The difference is the same for different types of defects.