Isolation And Identification Of Goose Source Candida Albicans And Establishment Of PCR Detection Method

Goose moniliasia,also known as fungal stomatitis or thrush,is a fungal infection of the goose’s upper digestive tract caused by Candida albicans.Candida albicans multi-invasion gosling,it is a common infectious disease in the growth and development of goslings,which seriously affects the healthy development of goose industry in China.Establishing a simple,fast and accurate detection method is of great significance for controlling Candida albicans’ s infection.In this research,three suspected strains were isolated from the geese suspected to be infected with Candida albicans,and then,three suspected strains were cultured from the esophageal esophagus,and identified by color culture and molecular biology.The results showed that the isolates were cultured in green color on Candida albicans chromogenic medium.Under microscope observation,the bacteriums were blue-purple oval-shaped yeast-like bacteria after Gram staining;after IST interval gene sequence analysis,The homology between the isolate and the published Candida albicans on Gen Bank is 86.1-87.1%.,and the homology with other Candida is 29.1 to 85.0%.Based on the above results,the isolate obtained in this research was identified as Candida albicans.A pair of specific primers with a length of 471 bp were designed according to the published nucleotide sequence of Candida albicans on Gen Bank.After optimization of conditions,a PCR detection method for Goose infected with Candida albicans was established.Sensitivity and repeatability evaluation test results of the method indicated that the minimum detection concentration of Candida albicans was 2.042 ng/μL,and the repeatability experiment results are consistent.The method is simple and convenient to operate,has low requirements on equipment conditions,can meet clinical testing needs,and has high practical value.This establishment of PCR detection method for Candida albicans provides a technical means forrapid diagnosis of Candida albicans infection,and also provides a research basis for the subsequent research on other detection methods of Candida albicans infection.