Identification And Expression Analysis During Germination Of SnRK2 Gene In Foxtail Millet

The objective of this research is to study the molecular mechanism of foxtail millet under drought stress during germination.In this study,Jin Gu 45 is the research material that was treated with 18%PEG-6000 and distilled water germinating for 10h and 18h,using the gene expression profiling technology to analysis gene expression and obtain the key gene of SnRK2.Then,SnRK2 gene family was identified and the expression pattern of these genes were analysised under PEG-6000、NaCl and ABA stress.The research results show that:1.456 and 545 genes screened out during millet germination at 10h and 18h,in which 87 and 267 DEGs were up-regulated and 369 and 278 DEGs were down-regulated.GO enrichment analysis showed that these DEGs were mainly relating to metabolism process,cell stimulation and response process.The KEGG enrichment analysis showed that these DEGs were associated with phenylpropanoid metabolism and plant hormone signal transduction.The results obtained from five genes tested by RT-PCR agreed with the trend of regulation identified by gene expression profile.2.10 SnRK2 genes in millet genome were identified,exon-intron of these genes distribute conservatively,most of them have 9 exons;SnRK2 gene was rich in acidic amino acids,10 protein sequences of the GRAVY values were negative,so they were hydrophilic protein,but their hydrophilic degree is not at same level.The cis element analysis show that upstream of promoter region contain different elements were relative to hormone and response to strss,the type and number of these genes were different.These results indicated that SnRK2 genes may have different functions in different hormones and environments.3.Overall,trend of relative expression level of SnRK2 gene family under PEG-6000,NaCl and ABA stress during the germination are same,it first increased and then decreased.SiSnRK2.3 was mainly responsed to drought stress during seed germination,SiSnRK2.1 was mainly responsed to ABA sress,SiSnRK2.3/1 were responsed to NaCl stress;SiSnRK2.l was responsed to these three kinds of exogenous environment,SiSnRK2.2 was induced by drought and ABA,SiSnRK2.4 was upregulated expression under ABA and NaCl stress,so SiSnRK2.1/2/4 dependent on ABA to play a role.